Pearson Correlation Analysis of Microarray Data Allows for the Identification of Genetic Targets for Early B-cell Factor[boxs]

• Published in: The Journal of biological chemistry Vol. 279; no. 17; pp. 17905 - 17913
• Main Authors: Månsson, Robert, Tsapogas, Panagiotis, Åkerlund, Mikael, Lagergren, Anna, Gisler, Ramiro, Sigvardsson, Mikael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.04.2004; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Antigens, CD19 - biosynthesis; B-Lymphocytes - metabolism; Binding Sites; Bone Marrow Cells - cytology; Cell Line; Dimerization; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene Expression Regulation; Hematopoietic Stem Cells - metabolism; Mice; Oligonucleotide Array Sequence Analysis; Oligonucleotides - chemistry; PAX5 Transcription Factor; Plasmids - metabolism; Polymerase Chain Reaction; Promoter Regions, Genetic; Protein Biosynthesis; Retroviridae - genetics; Reverse Transcriptase Polymerase Chain Reaction; Statistics as Topic; TECHNOLOGY; TEKNIKVETENSKAP; Trans-Activators - genetics; Transcription Factors - metabolism; Transcription, Genetic
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.M400589200

B lymphocyte development is a complex biological process critically dependent on the transcription factor early B cell factor (EBF). To deepen understanding of the roles for EBF in this process, we have used Pearson correlation analysis to evaluate microarray data from a set of mouse B lymphoid cell lines representing different stages of development. Comparing the expression pattern of EBF to that of the other genes in the data set revealed that VpreB1, mb-1, and λ5, all known target genes, presented high correlation values to EBF. High correlations were also seen for the VpreB3 and CD19 genes and biochemical as well as functional data supported that they are target genes for EBF even though the expression of CD19 was critically dependent of Pax-5. We also obtained evidence for extensive collaborative actions of EBF and E47 even though microarray analysis of hematopoetic progenitor cells ectopically expressing these proteins suggested that they activated only a subset of pre-B cell restricted genes.