Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing

• Published in: Journal of endodontics Vol. 39; no. 9; pp. 1136 - 1140
• Main Authors: Hong, Bo-Young, BS, MS, PhD, Lee, Tae-Kwon, BS, PhD, Lim, Sang-Min, DDS, MSD, Chang, Seok Woo, DDS, PhD, Park, Joonhong, BE, MEng, PhD, Han, Seung Hyun, PhD, Zhu, Qiang, DDS, PhD, Safavi, Kamran E., DMD, MEd, Fouad, Ashraf F., DDS, MD, Kum, Kee Yeon, DDS, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2013
• Subjects: Actinobacteria - classification; Adult; Aged; Asymptomatic Diseases; Bacteria - classification; Bacteroidetes - classification; Dental Pulp Cavity - microbiology; Dental Pulp Diseases - microbiology; Dentistry; Diversity; Endocrinology & Metabolism; Fusobacteria - classification; Fusobacterium - classification; Gram-Negative Anaerobic Bacteria - classification; Gram-Negative Bacteria - classification; Humans; Microbial Consortia; Middle Aged; operational taxonomic unit; Periapical Periodontitis - microbiology; persistent infection; Prevotella - classification; primary infection; Propionibacterium - classification; Proteobacteria - classification; pyrosequencing; richness; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; Sequence Analysis, DNA; Spirochaetales - classification; Young Adult; primary infection; pyrosequencing; richness; persistent infection; operational taxonomic unit; Diversity

Abstract Introduction The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that ther...