Microbial Analysis in Primary and Persistent Endodontic Infections by Using Pyrosequencing

• Published in: Journal of endodontics Vol. 39; no. 9; pp. 1136 - 1140
• Main Authors: Hong, Bo-Young, BS, MS, PhD, Lee, Tae-Kwon, BS, PhD, Lim, Sang-Min, DDS, MSD, Chang, Seok Woo, DDS, PhD, Park, Joonhong, BE, MEng, PhD, Han, Seung Hyun, PhD, Zhu, Qiang, DDS, PhD, Safavi, Kamran E., DMD, MEd, Fouad, Ashraf F., DDS, MD, Kum, Kee Yeon, DDS, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2013
• Subjects: Actinobacteria - classification; Adult; Aged; Asymptomatic Diseases; Bacteria - classification; Bacteroidetes - classification; Dental Pulp Cavity - microbiology; Dental Pulp Diseases - microbiology; Dentistry; Diversity; Endocrinology & Metabolism; Fusobacteria - classification; Fusobacterium - classification; Gram-Negative Anaerobic Bacteria - classification; Gram-Negative Bacteria - classification; Humans; Microbial Consortia; Middle Aged; operational taxonomic unit; Periapical Periodontitis - microbiology; persistent infection; Prevotella - classification; primary infection; Propionibacterium - classification; Proteobacteria - classification; pyrosequencing; richness; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; Sequence Analysis, DNA; Spirochaetales - classification; Young Adult; primary infection; pyrosequencing; richness; persistent infection; operational taxonomic unit; Diversity
• ISSN: 0099-2399; 1878-3554
• DOI: 10.1016/j.joen.2013.05.001

Abstract Introduction The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections. Methods Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted. Results Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups ( P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition ( P > .05). Conclusions The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.