The impact of ultraviolet B (UV-B) radiation in combination with different temperatures in the early life stage of zebrafish (Danio rerio)

• Published in: Photochemical & photobiological sciences Vol. 17; no. 1; pp. 35 - 41
• Main Authors: Icoglu Aksakal, Feyza, Ciltas, Abdulkadir
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.01.2018; Royal Society of Chemistry
• Subjects: Aquatic organisms; Biochemistry; Biomaterials; Catalase; Chemistry; Danio rerio; Developmental stages; Embryogenesis; Embryonic growth stage; Embryos; Environmental effects; Environmental factors; Environmental stress; Exposure; Fertilization; Genes; Hatching; Heart rate; Heat shock proteins; Hsp70 protein; Immune response; Immune system; Innate immunity; Interleukin 1; Larvae; Metabolism; mRNA; Oxidative stress; Physical Chemistry; Plant Sciences; Polymerase chain reaction; Radiation effects; Superoxide dismutase; Survival; Toxicity; Tumor necrosis factor-α; Ultraviolet radiation; Zebrafish
• ISSN: 1474-905X; 1474-9092
• DOI: 10.1039/c7pp00236j

Ultraviolet B (UV-B) radiation is an environmental stressor with detrimental effects on many aquatic organisms including fish. In addition, UV-B exposure combined with other environmental factors could have even more negative effects. The purpose of this study was to investigate the effect of UV-B radiation exposure on zebrafish embryos/larvae in terms of survival, developmental toxicity and the mRNA levels of the genes related to oxidative stress and innate immune response at different temperatures (24 °C, 28 °C and 30 °C). Zebrafish embryos were exposed to 3.3 W m −2 UV-B radiation and/or 24 °C, 28 °C (for the control) and 30 °C temperatures between 4 and 96 h post-fertilization. The mortality, hatching rate, malformations and heartbeat rate were evaluated. The results demonstrated that UV-B exposure or different temperatures (24 °C and 30 °C) induced developmental toxicity, including delayed hatching, increased the occurrence of malformations, and reduced the heartbeat rate and survival. The combined exposure to UV-B and different temperatures (24 °C and 30 °C) resulted in greater adverse effects on embryonic development. Furthermore, RT-PCR results showed that the mRNA levels of superoxide dismutase 1 ( sod1 ), catalase 1 ( cat1 ), heat shock protein 70 ( hsp70 ), interleukin-1 beta ( il-1β ) and tumor necrosis factor alpha ( tnfα ) genes were significantly up-regulated in all of the treatment groups. These results revealed that the interaction between UV-B and temperature impaired the development of zebrafish embryos and disrupted their metabolism. Ultraviolet B (UV-B) radiation is an environmental stressor with detrimental effects on many aquatic organisms including fish.