Methods and options for the heterologous production of complex natural products
Covering: 1990 to 2010 Heterologous biosynthesis has emerged as a viable route to access the beneficial properties of natural products. This development primarily owes to the difficulties encountered when using traditional methods of natural product discovery and production. However, the process of...
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| Published in | Natural product reports Vol. 28; no. 1; pp. 125 - 151 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.01.2011
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Covering: 1990 to 2010
Heterologous biosynthesis has emerged as a viable route to access the beneficial properties of natural products. This development primarily owes to the difficulties encountered when using traditional methods of natural product discovery and production. However, the process of heterologous biosynthesis also presents a number of challenges that have produced an array of creative solutions and noteworthy success stories. In this review, a historical perspective will be presented, together with an analysis of the experimental approaches thus far used to address the unique issues associated with heterologous natural product biosynthesis.
This review will detail the motivations, experimental approaches, and growing list of successful cases associated with the heterologous production of complex natural products. |
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| Bibliography: | John Armando is completing a dual B.S/M.S. degree at Tufts in chemical and biological engineering. His research is focused on further expanding the metabolic capabilities of heterologous host systems to better accommodate complex natural product pathways. as a heterologous host. After receiving his PhD in 2002, and following a postdoctoral position at MIT, he began as an assistant professor at Tufts University in the chemical and biological engineering department. Since that time, his laboratory has continued to work on problems related to heterologous natural product biosynthesis, with particular focus on successful gene transfer to and metabolic engineering of the recipient hosts. Blaine Pfeifer received his bachelor's degree in 1997 from Colorado State University in chemical engineering. He pursued graduate work the following year under the direction of Chaitan Khosla at Stanford University. His doctoral thesis focused upon the production of complex polyketide and nonribosomal peptide compounds using He has also made efforts to heterologously produce marine natural products using Haoran Zhang is completing his doctorate at Tufts University after having received his B.S. and M.S. degrees from Xiamen University. His research at Tufts has focused on establishing and optimizing the biosynthesis of erythromycin A from E. coli . Brett Boghigian is completing his doctoral research at Tufts University after having completed his B.S. and M.S., also at Tufts. His research has focused on applying metabolic and process engineering to the heterologous production of complex natural products. Specifically, he has combined computational and experimental approaches to rationally improving the heterologous production titers of both polyketide and isoprenoid natural products using as a surrogate host. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-2 |
| ISSN: | 0265-0568 1460-4752 |
| DOI: | 10.1039/c0np00037j |