Engineered microbial host selection for value-added bioproducts from lignocellulose

• Published in: Biotechnology advances Vol. 37; no. 6; p. 107347
• Main Authors: de Paula, Renato Graciano, Antoniêto, Amanda Cristina Campos, Ribeiro, Liliane Fraga Costa, Srivastava, Neha, O'Donovan, Anthonia, Mishra, P.K., Gupta, Vijai K., Silva, Roberto N.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.11.2019
• Subjects: Biofuels; Biomass; Hydrolysis; Lignin; Lignocellulosic biomass; Metabolic engineering; Protein engineering; Transcriptional factors; Value-added bioproducts; Glf; TracrRNA; Transcriptional factors; AraD; ptsG; AraB; AraA; galP; EpPCR; SREBPs; MDR; RBP; EG; glk; XBP; als; Metabolic engineering; Cas9; Tkt; NADPH; BG; ABF; CBH; 2,3-BDO; StEP; CBM; CBP; pforA; NADH; CAZYmes; GBP; HDR; LPMOs; Xyl A; Xyl B; XDH; HMF; Protein engineering; CRISPR; ldhA; XYG; aldC; bdh; Lignocellulosic biomass; AXE; CCR; XK; Value-added bioproducts; pflB; XR; NHEJ; PAM; Tal
• ISSN: 0734-9750; 1873-1899
• DOI: 10.1016/j.biotechadv.2019.02.003

Lignocellulose is a rich and sustainable globally available carbon source and is considered a prominent alternative raw material for producing biofuels and valuable chemical compounds. Enzymatic hydrolysis is one of the crucial steps of lignocellulose degradation. Cellulolytic and hemicellulolytic enzyme mixes produced by different microorganisms including filamentous fungi, yeasts and bacteria, are used to degrade the biomass to liberate monosaccharides and other compounds for fermentation or conversion to value-added products. During biomass pretreatment and degradation, toxic compounds are produced, and undesirable carbon catabolic repression (CCR) can occur. In order to solve this problem, microbial metabolic pathways and transcription factors involved have been investigated along with the application of protein engineering to optimize the biorefinery platform. Engineered Microorganisms have been used to produce specific enzymes to breakdown biomass polymers and metabolize sugars to produce ethanol as well other biochemical compounds. Protein engineering strategies have been used for modifying lignocellulolytic enzymes to overcome enzymatic limitations and improving both their production and functionality. Furthermore, promoters and transcription factors, which are key proteins in this process, are modified to promote microbial gene expression that allows a maximum performance of the hydrolytic enzymes for lignocellulosic degradation. The present review will present a critical discussion and highlight the aspects of the use of microorganisms to convert lignocellulose into value-added bioproduct as well combat the bottlenecks to make the biorefinery platform from lignocellulose attractive to the market.