Growth of Escherichia coli in Whole and Skim Milk from Endotoxin-Induced Mastitic Quarters: In Vitro Effects of Deferoxamine, Zinc, and Iron Supplementation

• Published in: Journal of dairy science Vol. 71; no. 10; pp. 2772 - 2781
• Main Authors: Lohuis, J.A.C.M., Van Leeuwen, W., Verheijden, J.H.M., Smit, J.A.H, Brand, A., Van Miert, A.S.J.P.A.M.
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.10.1988; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: Action of physical and chemical agents on bacteria; Animals; Bacteriology; Biological and medical sciences; BOVIN LAITIER; BOVINE MASTITIS; Cattle; CHELATEUR; CHELATING AGENTS; CINC; COWS; CRECIMIENTO; CROISSANCE; DAIRY CATTLE; Deferoxamine - pharmacology; ENDOTOXINAS; ENDOTOXINE; ENDOTOXINS; Endotoxins - toxicity; ESCHERICHIA; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - growth & development; EXPERIMENTACION IN VITRO; EXPERIMENTATION IN VITRO; Female; FER; Fundamental and applied biological sciences. Psychology; GANADO DE LECHE; GROWTH; HIERRO; IN VITRO EXPERIMENTATION; IRON; Iron - pharmacology; LAIT; LAIT ECREME; LECHE; LECHE DESNATADA; MAMMITE BOVINE; MASTITIS BOVINA; Mastitis, Bovine - chemically induced; Mastitis, Bovine - physiopathology; Microbiology; MILK; Milk - drug effects; QUELATOS; SKIM MILK; TEMPERATURA; TEMPERATURE; VACA; VACHE; ZINC; Zinc - pharmacology; Growth inhibitor; Mastitis; Microorganism growth; Growth rate; Escherichia coli; Ox; Iron; Cow; Zinc; Vertebrata; Mammalia; Bacteria; Cow milk; Artiodactyla; Biological effect; Mammary gland; Escherichieae; Ungulata; Enterobacteriaceae
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(88)79871-9

A marked growth inhibition of Escherichia coli 0101 K99 F41 was observed in whole and skim milk collected from inflamed quarters 18 and 36h after intramammary administration of .1mg E. coli lipopolysaccharide. Individual cow variation in the ability of milk from endotoxin-infused quarters to inhibit growth of E. coli was found. Growth inhibition of E. coli was observed in milk from endotoxin-infused quarters and was most pronounced in skim milk sampled at postinfusion h 18, and incubated at 38°C. The mechanism by which bacterial growth was depressed was probably of noncellular origin. Addition of Fe (45.5μg/ml) and Zn (2.7μg/ml) to whole and skim milk sampled from inflamed quarters at 18h after endotoxin infusion resulted in a growth-promoting effect. Addition of deferoxamine (6 mg/ml) depressed bacterial growth. Effects of Fe, Zn, and deferoxamine on bacterial growth did not differ in whole and skim milk. No clear relationship was observed between reduction in Zn concentrations in skim milk from inflamed quarters at 18h after endotoxin infusion and growth inhibition of E. coli in the same samples.