X‐ray fluorescence detection for serial macromolecular crystallography using a JUNGFRAU pixel detector

Detection of heavy elements, such as metals, in macromolecular crystallography (MX) samples by X‐ray fluorescence is a function traditionally covered at synchrotron MX beamlines by silicon drift detectors, which cannot be used at X‐ray free‐electron lasers because of the very short duration of the X...

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Published inJournal of synchrotron radiation Vol. 27; no. 2; pp. 329 - 339
Main Authors Martiel, Isabelle, Mozzanica, Aldo, Opara, Nadia L., Panepucci, Ezequiel, Leonarski, Filip, Redford, Sophie, Mohacsi, Istvan, Guzenko, Vitaliy, Ozerov, Dmitry, Padeste, Celestino, Schmitt, Bernd, Pedrini, Bill, Wang, Meitian
Format Journal Article
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.03.2020
John Wiley & Sons, Inc
Subjects
Crystallography
Fluorescence
Heavy elements
JUNGFRAU detector
macromolecular crystallography
Pixels
Research Papers
serial crystallography
silicon drift detectors
XFELs
silicon drift detectors
serial crystallography
fluorescence
XFELs
macromolecular crystallography
JUNGFRAU detector
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Summary:Detection of heavy elements, such as metals, in macromolecular crystallography (MX) samples by X‐ray fluorescence is a function traditionally covered at synchrotron MX beamlines by silicon drift detectors, which cannot be used at X‐ray free‐electron lasers because of the very short duration of the X‐ray pulses. Here it is shown that the hybrid pixel charge‐integrating detector JUNGFRAU can fulfill this function when operating in a low‐flux regime. The feasibility of precise position determination of micrometre‐sized metal marks is also demonstrated, to be used as fiducials for offline prelocation in serial crystallography experiments, based on the specific fluorescence signal measured with JUNGFRAU, both at the synchrotron and at SwissFEL. Finally, the measurement of elemental absorption edges at a synchrotron beamline using JUNGFRAU is also demonstrated. Use of the charge‐integrating JUNGFRAU detector for fluorescence detection is investigated, and its applications in macromolecular crystallography at synchrotron and X‐ray free‐electron laser sources are demonstrated.
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ISSN:1600-5775
0909-0495
1600-5775
DOI:10.1107/S1600577519016758