Bromomaleimide-Linked Bioconjugates Are Cleavable in Mammalian Cells

Bromomaleimides are versatile scaffolds that allow facile conjugation of thiolated biomolecules. Here we demonstrate that bromomaleimide‐linked GFP–rhodamine FRET pairs cleave in the cytoplasm of mammalian cells. We believe that bromomaleimide scaffolds provide a potential core structure for prodrug...

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Published inChembiochem : a European journal of chemical biology Vol. 13; no. 1; pp. 39 - 41
Main Authors Moody, Paul, Smith, Mark. E. B., Ryan, Chris P., Chudasama, Vijay, Baker, James R., Molloy, Justin, Caddick, Stephen
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 02.01.2012
WILEY‐VCH Verlag
Subjects
Communications
drug delivery
fluorescent probes
Green Fluorescent Proteins - chemistry
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
in-cell cleavage
Kinetics
Maleimides - chemistry
Maleimides - metabolism
microinjection
Molecular Structure
protein modifications
Rhodamines - chemistry
Rhodamines - metabolism
Time Factors
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Summary:Bromomaleimides are versatile scaffolds that allow facile conjugation of thiolated biomolecules. Here we demonstrate that bromomaleimide‐linked GFP–rhodamine FRET pairs cleave in the cytoplasm of mammalian cells. We believe that bromomaleimide scaffolds provide a potential core structure for prodrugs designed to release bioactive cargo following cell internalisation.
Bibliography:RCUK
Funded Access
UCLB
BBSRC
ArticleID:CBIC201100603
ark:/67375/WNG-JJ8B4SCG-H
EPSRC
istex:6FE8CBD512F0646CE3696842FBBE886201514CF1
Wellcome Trust
MRC
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Supporting information for this article is available on the WWW under http://dx.doi.org/10.1002/cbic.201100603.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201100603