Functional TASK-3–Like Channels in Mitochondria of Aldosterone-Producing Zona Glomerulosa Cells

• Published in: Hypertension (Dallas, Tex. 1979) Vol. 70; no. 2; pp. 347 - 356
• Main Authors: Yao, Junlan, McHedlishvili, David, McIntire, William E, Guagliardo, Nick A, Erisir, Alev, Coburn, Craig A, Santarelli, Vincent P, Bayliss, Douglas A, Barrett, Paula Q
• Format: Journal Article
• Language: English
• Published: United States American Heart Association, Inc 01.08.2017
• Subjects: Aldosterone - biosynthesis; Cells, Cultured; Humans; Hyperaldosteronism - metabolism; Membrane Potential, Mitochondrial - physiology; Mitochondria - physiology; Potassium Channels, Tandem Pore Domain - physiology; Zona Glomerulosa - physiology; hyperaldosteronism; zona glomerulosa; aldosterone; mitochondria; ion channels
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/HYPERTENSIONAHA.116.08871

Ca drives aldosterone synthesis in the cytosolic and mitochondrial compartments of the adrenal zona glomerulosa cell. Membrane potential across each of these compartments regulates the amplitude of the Ca signal; yet, only plasma membrane ion channels and their role in regulating cell membrane potential have garnered investigative attention as pathological causes of human hyperaldosteronism. Previously, we reported that genetic deletion of TASK-3 channels (tandem pore domain acid-sensitive K channels) from mice produces aldosterone excess in the absence of a change in the cell membrane potential of zona glomerulosa cells. Here, we report using yeast 2-hybrid, immunoprecipitation, and electron microscopic analyses that TASK-3 channels are resident in mitochondria, where they regulate mitochondrial morphology, mitochondrial membrane potential, and aldosterone production. This study provides proof of principle that mitochondrial K channels, by modulating inner mitochondrial membrane morphology and mitochondrial membrane potential, have the ability to play a pathological role in aldosterone dysregulation in steroidogenic cells.