Boron-Based Inhibitors of Acyl Protein Thioesterases 1 and 2

• Published in: Chembiochem : a European journal of chemical biology Vol. 14; no. 1; pp. 115 - 122
• Main Authors: Zimmermann, Tobias J., Bürger, Marco, Tashiro, Etsu, Kondoh, Yasumitsu, Martinez, Nancy E., Görmer, Kristina, Rosin-Steiner, Sigrid, Shimizu, Takeshi, Ozaki, Shoichiro, Mikoshiba, Katsuhiko, Watanabe, Nobumoto, Hall, Dennis, Vetter, Ingrid R., Osada, Hiroyuki, Hedberg, Christian, Waldmann, Herbert
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 02.01.2013; WILEY‐VCH Verlag; Wiley; Wiley Subscription Services, Inc
• Subjects: acyl protein thioesterases; Animals; Biochemistry & Molecular Biology; Boron; Boron - chemistry; Boron - pharmacology; Boron - toxicity; boronic acids; Chemistry, Medicinal; Dogs; Drug Evaluation, Preclinical; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Enzyme Inhibitors - toxicity; Humans; inhibitors; Life Sciences & Biomedicine; Lipoylation - drug effects; Madin Darby Canine Kidney Cells; microarrays; oncogenic Ras proteins; Pharmacology & Pharmacy; ras Proteins - antagonists & inhibitors; ras Proteins - metabolism; Science & Technology; Thiolester Hydrolases - antagonists & inhibitors; TARGET; inhibitors; LOCALIZATION; SUBTILISIN; RECOGNITION; RAS; ACIDS; IDENTIFICATION; microarrays; PEPTIDES; DISEASES; oncogenic Ras proteins; acyl protein thioesterases; SMALL-MOLECULE INHIBITION; boronic acids
• ISSN: 1439-4227; 1439-7633; 1439-7633
• DOI: 10.1002/cbic.201200571

Ras proteins are of importance in cell proliferation, and hence their mutated forms play causative roles in many kinds of cancer in different tissues. Inhibition of the Ras‐depalmitoylating enzyme acyl protein thioesterases APT1 and ‐2 is a new approach to modulating the Ras cycle. Here we present boronic and borinic acid derivatives as a new class of potent and nontoxic APT inhibitors. These compounds were detected by extensive library screening using chemical arrays and turned out to inhibit human APT1 and ‐2 in a competitive mode. Furthermore, one of the molecules was demonstrated to inhibit Erk1/2 phosphorylation significantly. Ras depalmitoylation inhibitors: Different boronic and boronic acid inhibitors were found to target acyl protein thioesterases on a microarray screening chip. Individual inhibitors also display appreciable isoenzymatic specificity for APT2; this makes these boronic acids a class of APT inhibitors with specificity for one of the APTs.