Alginate as immobilization material. III. Diffusional properties

The rate of diffusion of serum albumin (MW 6.9.10(4) D) out of beads of calcium alginate gels depends upon the concentration and uronic acid composition of the alginate (ManA/GulA ratio), the conditions under which the beads are produced, the pH, and the temperature. The diffusion coefficient decrea...

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Bibliographic Details
Published inBiotechnology and bioengineering Vol. 39; no. 2; pp. 186 - 194
Main Authors Martinsen, A, Storro, I, Skjak-Braek, G
Format Journal Article
LanguageEnglish
Published United States 20.01.1992
Subjects
alginates
bovine serum albumin
calcium alginate
diffusion
gels
gulutonic acid
immobilization
immobilized cells
immobilized enzymes
Laminaria
laminaria digitata
laminaria hyperborea
macrocystis pyrifera
mannuronic acid
Phaeophycophyta
uronic acids
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Summary:The rate of diffusion of serum albumin (MW 6.9.10(4) D) out of beads of calcium alginate gels depends upon the concentration and uronic acid composition of the alginate (ManA/GulA ratio), the conditions under which the beads are produced, the pH, and the temperature. The diffusion coefficient decreases with increasing alginate concentration, and (ManA/GulA) ratio and with decreasing pH. Diffusion out of the beads, in which the alginate is uniformly distributed (homogeneous gel), is faster than out of the beads in which the alginate is concentrated at the surface (inhomogeneous gel). The temperature dependence of the diffusion coefficient follows the Arrhenius law, with an activation energy of approximately 23 kJ.mol-1.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260390210