Comparison of One-Day Versus One-Hour Application of Topical Gatifloxacin in Eliminating Conjunctival Bacterial Flora

• Published in: Ophthalmology (Rochester, Minn.) Vol. 115; no. 11; pp. 2013 - 2016
• Main Authors: Moss, Jason M., BA, Nguyen, David, BS, Liu, Yueyi I., PhD, Singh, Kuldev, MD, MPH, Montague, Artis, MD, PhD, Egbert, Peter R., MD, Kaspar, Herminia Mino De, PhD, Ta, Christopher N., MD
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.11.2008; Elsevier
• Subjects: Administration, Topical; Aged; Anti-Infective Agents, Local - administration & dosage; Bacteria; Bacteria - drug effects; Bacteria - growth & development; Bacteriological Techniques; Biological and medical sciences; Cataract Extraction; Colony Count, Microbial; Conjunctiva - microbiology; Female; Fluoroquinolones - administration & dosage; Humans; Keratoplasty, Penetrating; Male; Medical sciences; Miscellaneous; Ophthalmology; Prospective Studies; Time Factors; Infection; Local administration; Fluoroquinolone derivatives; Gatifloxacin; Bacteriosis; Bacteria; Ophthalmology; Antibacterial agent; Conjunctiva; Eradication; Quinolone derivatives; Comparative study
• ISSN: 0161-6420; 1549-4713
• DOI: 10.1016/j.ophtha.2008.06.024

Purpose To compare efficacies of 1-day, 1-hour, and combined 1-day/1-hour preoperative topical gatifloxacin in eliminating conjunctival bacterial flora. Design Prospective, comparative case series. Participants Sixty patients (120 eyes) scheduled to undergo anterior segment intraocular surgery at Stanford University Medical Center. Methods Cultures were collected from the palpebral conjunctival sac at baseline and after 1 day (4 doses), 1 hour (3 doses), and 1 day/1 hour (7 doses) of gatifloxacin use. Main Outcome Measures Incidence of positive bacterial samples collected pre- and post-antibiotic treatment and number of colony forming units (CFUs). Results SeptiChek (Becton Dickinson, Franklin Lakes, NJ) positive cultures significantly decreased from 67% growth at baseline to 28% ( P <0.0001) after 1 day and from 60% at baseline to 37% ( P = 0.018) after 1 hour of gatifloxacin use. Reductions of 44% growth at baseline to 12% ( P = 0.0001) after 1 day and 32% at baseline to 13% ( P = 0.029) after 1 hour of gatifloxacin use were observed on blood agar. Surgical eyes that received both 1-day and 1-hour preoperative gatifloxacin had reductions from 67% growth at baseline to 18% posttreatment ( P <0.0001) and 45% at baseline to 7% posttreatment ( P <0.0001) on SeptiChek and blood agar media, respectively. In addition to a lower frequency of positive cultures, a significantly lower CFU count was found after 1-day ( P = 0.004) and 1-hour ( P = 0.049) gatifloxacin use compared with pretreatment levels. Combined 1-day/1-hour doses of gatifloxacin were associated with a greater reduction in CFUs ( P = 0.001) when compared with 1-hour treatment alone. Conclusions Both 1-hour and 1-day topical gatifloxacin use are effective in reducing the frequency of conjunctival bacterial growth and the overall bacterial load as measured by CFUs, relative to baseline. Although a 1-hour pretreatment is associated with a reduction in bacterial growth, the combination of 1-day and 1-hour preoperative gatifloxacin dosing results in an even lower overall bacterial load, suggesting that the latter might be the preferred preoperative regimen for eyes undergoing anterior segment surgery. Financial Disclosure(s) Proprietary or commercial disclosure may be found after the references.