Expressions of Iba1 and galectin-3 (Gal-3) in thioacetamide (TAA)-induced acute rat liver lesions

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie Vol. 65; no. 6; pp. 799 - 808
• Main Authors: Wijesundera, Kavindra Kumara, Juniantito, Vetnizah, Golbar, Hossain M., Fujisawa, Kae, Tanaka, Miyuu, Ichikawa, Chisa, Izawa, Takeshi, Kuwamura, Mitsuru, Yamate, Jyoji
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2013
• Subjects: Acute Disease; Animals; body weight; calcium; Calcium-Binding Proteins - biosynthesis; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; Fibrosis; Fluorescent Antibody Technique; Galectin 3 - biosynthesis; Galectin-3; Hepatotoxicity; Iba1; Immunohistochemistry; ionization; lectins; liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Macrophages; Macrophages - drug effects; Macrophages - metabolism; Male; messenger RNA; Microfilament Proteins - biosynthesis; Microscopy, Confocal; muscles; pathogenesis; phagocytosis; Rats; Rats, Sprague-Dawley; Real-Time Polymerase Chain Reaction; reverse transcriptase polymerase chain reaction; Thioacetamide - toxicity; Iba1; Galectin-3; Macrophages; Hepatotoxicity; Fibrosis
• ISSN: 0940-2993; 1618-1433
• DOI: 10.1016/j.etp.2012.11.006

Ionized calcium binding adaptor molecule 1 (Iba1) is associated with membrane ruffling and motility of cells. Galectin-3 (Gal-3) is a β-galactoside binding animal lectin, and regulates fibrogenesis probably through transforming growth factor-β1. To evaluate macrophage properties, expressions of Iba1 and Gal-3 were investigated, in relation to macrophages expressing CD68 (ED1; reflecting increased phagocytosis) and CD163 (ED2; implying proinflammatory factor productions) in centrilobular lesions induced in rat livers with thioacetamide (TAA; 300mg/kg body weight, once intraperitoneally). In agreement with expression patterns of CD68+ and CD163+ macrophages, cells reacting to Iba1 and Gal-3 were increased in numbers on post-injection (PI) days 1–5, peaking on day 2; thereafter, the positive cells gradually decreased to control levels until PI days 7 and 10. The increased expressions of Iba1 and Gal-3 were confirmed at mRNA levels by the RT-PCR. Double immunofluorescence staining on PI days 2 and 3 demonstrated Iba1 expression in 15–46% of CD68+ and CD163+ macrophages, and Gal-3 expression in 65–82% of CD68+ and CD163+ macrophages; Gal-3 expression was observed in 84–93% of Iba1+ cells. Interestingly, Gal-3 was also expressed in a small number of α-smooth muscle actin-positive myofibroblasts in fibrotic lesions developed in injured centrilobular areas. These findings indicate that macrophages with various functions can participate in development of liver lesions and resultant fibrosis. Besides CD68 and CD163, Iba1 and Gal-3 immunohistochemistry for macrophages would be useful to analyze the pathogenesis behind developing hepatotoxicity.