Clinicopathological significance of missing in metastasis B expression in hepatocellular carcinoma

• Published in: Human pathology Vol. 38; no. 8; pp. 1201 - 1206
• Main Authors: Ma, Stephanie, MSc, Guan, Xin-Yuan, PhD, Lee, Terence K., PhD, Chan, Kwok Wah, MBBS
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.08.2007; Elsevier; Elsevier Limited
• Subjects: Adult; Aged; Biological and medical sciences; Biomarkers, Tumor - metabolism; Blotting, Western; Cancer; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - secondary; Female; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression; Hepatitis; Hepatocellular carcinoma; Humans; Investigative techniques, diagnostic techniques (general aspects); Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Microfilament Proteins - genetics; Microfilament Proteins - metabolism; Middle Aged; MIM-B; Mortality; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Neoplasm Staging; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proteins; Real-time quantitative PC; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; RNA, Neoplasm - analysis; Rodents; Tumors; Hepatocellular carcinoma; MIM-B; Real-time quantitative PC; Liver cancer; Anatomic pathology; Metastasis suppressor gene; Digestive diseases; Hepatic disease; Malignant tumor; Metastasis; Real time; Quantitative analysis
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2007.01.004

Summary Missing in metastasis (MIM) proteins are important regulators in controlling cell growth and development. There has been accumulating evidence suggesting a role of MIM-B in carcinogenesis, yet its role in the development of hepatocellular carcinoma has not been examined thus far. In this study, we investigated the clinicopathological significance of MIM-B in tumor and its matched adjacent nontumor tissue obtained from 40 patients with hepatocellular carcinoma. Increased MIM-B messenger RNA and protein expression, as detected by quantitative real-time polymerase chain reaction and Western blot, respectively, was found in hepatocellular carcinoma clinical samples; and its expression was significantly associated with early pathologic tumor-node-metastasis stage group ( P = .007), presence of tumor encapsulation ( P = .034), and absence of venous infiltration ( P = .038). Higher levels of MIM-B expression were found to be associated with early stage disease. Elevated MIM-B expression may influence the development of hepatocellular carcinoma and may possibly be a powerful indicator for the disease at an early stage.