The promoter of the endo A cytokeratin gene is activated by a 3' downstream enhancer

• Published in: Nucleic acids research Vol. 19; no. 10; pp. 2761 - 2765
• Main Authors: TAKEMOTO, Y, FUJIMURA, Y, MATSUMOTO, M, TAMAI, Y, MORITA, T, MATSUSHIRO, A, NOZAKI, M
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25.05.1991
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cells, Cultured; Cloning, Molecular; DNA; Enhancer Elements, Genetic; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Keratins - genetics; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Polyomavirus; Promoter Regions, Genetic; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Simian virus 40; Teratoma; Transcription. Transcription factor. Splicing. Rna processing; Transfection; Tumor Cells, Cultured; Keratin; Vertebrata; Regulation(control); Enhancer sequence; Mammalia; Intermediary filament; Mouse; Transcription promoter; Rodentia; Homology; Regulatory sequence; Hybrid gene
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/19.10.2761

Mouse cytokeratin EndoA is an intermediate filament subunit of the type II cytokeratin class which initiates expression in trophectoderm cells of blastocyst during embryogenesis. To identify the regulatory elements of the endo A gene, we constructed a series of CAT expression vectors and transfected them into PYS-2 cells. We found an enhancer element locating 1 kb downstream from the endo A gene which acts on both the endo A and SV40 promoters. This enhancer consists of six direct repeated sequences with homology to the PEA3 motif in polyoma virus alpha enhancer core. In undifferentiated F9 embryonal carcinoma cells, expression of the construct containing the enhancer was not detected. These results indicate that one of the regulatory mechanisms of endo A gene expression is the 3' downstream enhancer.