Combination of probiotics with different functions alleviate DSS-induced colitis by regulating intestinal microbiota, IL-10, and barrier function

• Published in: Applied microbiology and biotechnology Vol. 104; no. 1; pp. 335 - 349
• Main Authors: Wang, Yanbo, Xie, Qiuhong, Zhang, Ying, Ma, Wen, Ning, Ke, Xiang, Jun-Yan, Cui, Jingwen, Xiang, Hongyu
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2020; Springer; Springer Nature B.V
• Subjects: Analysis; Anti-inflammatory agents; Applied Microbial and Cell Physiology; Bacteria; Biomedical and Life Sciences; Biotechnology; Colon; Dextran; Dextran sulfate; Dysbacteriosis; Herbal medicine; Histopathology; Immunology; Inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; Interleukin 10; Intestinal microflora; Intestine; Life Sciences; Maintenance; Microbial Genetics and Genomics; Microbiology; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Mucosa; Neomycin; Niches; Physicians; Physiological aspects; Probiotics; Sodium; Species; Sulfasalazine; Synergistic effect; Ulcerative colitis; China; Synergistic effects; Intestinal barrier; Immunological niches; Mucosal microbial ecology; Multi-strain probiotics; IL-10
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-019-10259-6

The potential of probiotics for treating ulcerative colitis (UC) has attracted increasing attention. However, more studies are still needed to guide physicians on the proper selection and use of probiotics. Here, we propose that combination of multiple probiotics with different functions can reduce intestinal inflammation. In this study, the effects of probiotics ( Lactobacillus reuteri , Bacillus coagulans , Bifidobacterium longum , and Clostridium butyricum ) on the physiology and histopathology of colon were evaluated in a dextran sulfate sodium (DSS)-induced colitis mouse model. The combined species, as well as the species individually, were tested and compared with sulfasalazine (SASP) and two Chinese herbal therapies. Results show that the functions of the four probiotic strains were different in regulating intestinal immunity and barrier function. The four-species probiotic cocktail was more effective than the species individually and anti-inflammatory drugs in repairing the dysbiosis of mucosal microbial ecology and reducing intestinal inflammation. The multi-strain probiotic mixture increased the proportion of beneficial bacteria and decreased the proportion of pro-inflammatory bacteria in the colonic mucosa. In addition, probiotic mixture significantly enhanced the expression of IL-10 and intestinal barrier function. These results suggest that a combination of multiple probiotics with different functions has synergistic effects and can restore the balance of interactions between microorganisms and immunological niches.