SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes
Development is orchestrated through a complex interplay of multiple transcription factors. The comprehension of this interplay will help us to understand developmental processes. Here we analyze the relationship between two key transcription factors: CBX4, a member of the Polycomb Repressive Complex...
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Published in | Frontiers in cell and developmental biology Vol. 9; p. 715868 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers media
21.09.2021
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Development is orchestrated through a complex interplay of multiple transcription factors. The comprehension of this interplay will help us to understand developmental processes. Here we analyze the relationship between two key transcription factors: CBX4, a member of the Polycomb Repressive Complex 1 (PRC1), and SALL1, a member of the Spalt-like family with important roles in embryogenesis and limb development. Both proteins localize to nuclear bodies and are modified by the small ubiquitin-like modifier (SUMO). Our results show that CBX4 and SALL1 interact in the nucleoplasm and that increased SALL1 expression reduces ubiquitination of CBX4, enhancing its stability. This is accompanied by an increase in the number and size of CBX4-containing Polycomb bodies, and by a greater repression of CBX4 target genes. Thus, our findings uncover a new way of SALL1-mediated regulation of Polycomb bodies through modulation of CBX4 stability, with consequences in the regulation of its target genes, which could have an impact in cell differentiation and development. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Ana Carmena, Institute of Neurosciences of Alicante (IN), Spain; Smita Misra, Meharry Medical College, United States; Stefan Müller, Goethe University Frankfurt, Germany These authors share first authorship Edited by: Juan Jose Sanz-Ezquerro, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Spain This article was submitted to Signaling, a section of the journal Frontiers in Cell and Developmental Biology Present address: Lucia Pirone, Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification, Rome, Italy |
ISSN: | 2296-634X 2296-634X |
DOI: | 10.3389/fcell.2021.715868 |