Resveratrol Inhibits IgE-Mediated Basophilic Mast Cell Degranulation and Passive Cutaneous Anaphylaxis in Mice

• Published in: The Journal of nutrition Vol. 143; no. 5; pp. 632 - 639
• Main Authors: Han, Seon-Young, Bae, Ji-Young, Park, Sin-Hye, Kim, Yun-Ho, Park, Jung Han Yoon, Kang, Young-Hee
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.05.2013; American Society for Nutrition
• Subjects: 3-(4,5-Dimetylthiazol-yl)-diphenyl tetrazolium bromide; Anaphylaxis - immunology; Anaphylaxis - metabolism; Anaphylaxis - prevention & control; Animals; Basophils; beta-N-Acetylhexosaminidases - metabolism; Biological and medical sciences; CD24 Antigen - metabolism; Chemokine CCL2 - metabolism; Chemokine CXCL2 - metabolism; Dietary Supplements; Dinitrophenols; dinitrophenyl; DNP; Dose-Response Relationship, Drug; Edema - immunology; Edema - prevention & control; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Histamine - metabolism; Histamine Release - drug effects; HSA; human serum albumin; IgE; immunoglobulin E; Immunoglobulin E - metabolism; Inflammation - prevention & control; Intracellular Signaling Peptides and Proteins - metabolism; macrophage inflammatory protein-2; Male; Mast Cells - drug effects; Mast Cells - metabolism; MCP-1; Mice; Mice, Inbred BALB C; MIP-2; monocyte chemotactic protein-1; MTT; Passive Cutaneous Anaphylaxis; PCA; Phospholipase C gamma - metabolism; phospholipase Cγ; Phosphorylation; Phytotherapy; piperazine-N,N'-bis(2-ethanesulfonic acid); PIPES; PKC; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; PLCγ; protein kinase C; Protein-Tyrosine Kinases - metabolism; Rats; reactive oxygen species; Resveratrol; ROS; Serum Albumin; Skin - drug effects; Skin - immunology; Skin - metabolism; spleen tyrosine kinase; Stilbenes - pharmacology; Stilbenes - therapeutic use; Syk; Syk Kinase; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitis - chemistry; MCP-1; Syk; IgE; MIP-2; DNP; HSA; ROS; PKC; PLCγ; MTT; PIPES; PCA; Nutrition; Rodentia; Micronutrient; Polyphenol; Vertebrata; Mammalia; Mouse; Animal; Resveratrol; Degranulation; Proanthocyanidin; Passive cutaneous anaphylaxis; Mast cell
• ISSN: 0022-3166; 1541-6100
• DOI: 10.3945/jn.112.173302

Resveratrol is a phytoalexin abundantly found in red grape skin and is effective in antitumor and antiinflammation associated with immune responses. This study investigated whether resveratrol suppressed immunoglobulin (Ig)E-mediated allergic responses and passive cutaneous anaphylaxis (PCA) in rat RBL-2H3 mast cells and in BALB/c mice. The release of β-hexosaminidase and histamine was enhanced in mast cells sensitized with anti-dinitrophenyl (DNP)-IgE and subsequently stimulated by DNP-human serum albumin (HSA), indicative of mast cell degranulation. When mast cells were pretreated with nontoxic resveratrol at 1–25 μmol/L, such induction was dose dependently diminished. Spleen tyrosine kinase (Syk) and phospholipase Cγ (PLCγ) of sensitized mast cells were activated by stimulation with DNP-HSA antigen, which was dampened by ≥5 μmol/L resveratrol. The phosphorylation of protein kinase C (PKC)μ and PKCθ was attenuated by administering resveratrol to DNP-HSA–exposed mast cells, whereas quiescent PKCζ/λ in sensitized cells was dose-dependently activated by resveratrol. Male BALB/c mice were sensitized for 24 h with DNP-IgE and orally administered with resveratrol 1 h before the DNP-HSA challenge. The histamine concentration was enhanced in sensitized mice challenged to DNP-HSA, which was reversed by administration of 10 mg/kg resveratrol. Additionally, it encumbered the tissue activation of Syk, PLCγ, and PKCμ in antigen-exposed mice. Resveratrol decreased IgE-mediated PCA and alleviated allergic edema of mouse ear and dorsal skin. Mast cell degranulation and allergic inflammation, accompanying the induction of monocyte chemotactic protein-1 and macrophage inflammatory protein-2, were inhibited by supplementing resveratrol to antigen-challenged mice. Resveratrol inhibited mast cell-derived, immediate-type allergic reactions, and these responses of resveratrol suggest possible therapeutic strategies in preventing allergic inflammatory diseases.