Sequence analysis of VP1 and VP7 genes suggests occurrence of a reassortant of G2 rotavirus responsible for an epidemic of gastroenteritis

• Published in: Journal of general virology Vol. 80; no. 6; pp. 1407 - 1415
• Main Authors: Zao, CL, Yu, WN, Kao, CL, Taniguchi, K, Lee, CY, Lee, CN
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.06.1999
• Subjects: Amino Acid Sequence; Antigens, Viral; Blotting, Northern; Capsid - genetics; Capsid Proteins; Disease Outbreaks; Gastroenteritis - epidemiology; Gastroenteritis - virology; Genes, Viral; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Reassortant Viruses - genetics; Rotavirus; Rotavirus - classification; Rotavirus - genetics; Rotavirus - isolation & purification; Rotavirus G2; Rotavirus Infections - epidemiology; Rotavirus Infections - virology; Sequence Analysis, DNA; Taiwan
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-80-6-1407

CL Zao, WN Yu, CL Kao, K Taniguchi, CY Lee and CN Lee School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan 100, Republic of China G23 rotavirus was prevalent in a 1993 epidemic of acute gastroenteritis in Taiwan. In this study, the genetic relationship among G2 rotavirus strains was analysed. The VP7 genes were amplified and sequenced. Except for one strain isolated in 1981, the nucleotide sequences of the VP7 genes of most of the G2 rotaviruses were very similar (identity >97%) and were closely related to that of a Japanese G2 reference strain, S2. The genetic relatedness of G2 rotaviruses was analysed further by RNA--RNA hybridization. The genomes of the major G2 strains of 1993 did not hybridize well with those of the G2 strains of previous seasons in RNA segments 1, 6 and 7. Partial nucleotide sequences of the VP1 gene were analysed and appeared to be similar among the major G2 strains from the same epidemic (identity >98%), whereas the identity of the VP1 genes of the major G2 strains of the 1993 epidemic to those of previous seasons was only about 84%. Since the numbers of mutations accumulated in the VP1 and VP7 genes over a period of 10 years were comparable, the significant change in the VP1 genes of the major strains of the 1993 epidemic suggests that these G2 rotaviruses had evolved by genetic reassortment.