Spontaneous aggregation of locust lipophorin during hemolymph collection

Density-gradient ultracentrifugation, gel permeation chromatography, polyacrylamide gel electrophoresis and electron microscopy were used to provide an experimental basis for the proposal that locust lipophorin aggregates spontaneously during the collection of hemolymph through a puncture in the nec...

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Bibliographic Details
Published inInsect biochemistry Vol. 17; no. 1; pp. 89 - 97
Main Authors Chino, Haruo, Hirayama, Yuji, Kiyomoto, Yukari, Downer, Roger G.H., Takahashi, Koui
Format Journal Article
LanguageEnglish
Published Oxford Elsevier B.V 1987
New York, NY Pergamon Press
Subjects
Biochemistry. Physiology. Immunology
Biological and medical sciences
collection
Fundamental and applied biological sciences. Psychology
hemolymph
Insecta
Invertebrates
lipoproteins
Locusta migratoria
Physiology. Development
Locust lipophorin
aggregation
hemolymph collection method
adipokinetic hormone
Gel electrophoresis
Insecta
Orthoptera
Hemolymph
Lipids
Locusta migratoria
Ultracentrifugation
Lipoprotein
Gel permeation chromatography
Acrididae
Aggregation
Proteins
Arthropoda
Density gradient
Invertebrata
Adipokinetic hormone
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Summary:Density-gradient ultracentrifugation, gel permeation chromatography, polyacrylamide gel electrophoresis and electron microscopy were used to provide an experimental basis for the proposal that locust lipophorin aggregates spontaneously during the collection of hemolymph through a puncture in the neck or coxal region of the insect. The evidence presented in this paper supports the above proposal and clearly demonstrates that the “puncture” method promotes the aggregation of lipophorin molecules and results in the formation of large aggregates as an artificial product. Thus, the use of this method should be discouraged for the study of lipophorin in locusts.
ISSN:0020-1790
DOI:10.1016/0020-1790(87)90148-X