GM3 synthase deficiency increases brain glucose metabolism in mice

• Published in: Molecular genetics and metabolism Vol. 137; no. 4; pp. 342 - 348
• Main Authors: Bharathi, Sivakama S., Zhang, Bob B., Paul, Eli, Zhang, Yuxun, Schmidt, Alexandra V., Fowler, Benjamin, Wu, Yijen, Tiemeyer, Michael, Inamori, Kei-ichiro, Inokuchi, Jin-ichi, Goetzman, Eric S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2022
• Subjects: Animals; Brain - diagnostic imaging; Brain - metabolism; G(M3) Ganglioside - metabolism; Ganglioside; Glucose - metabolism; GM3; Mice; Mice, Knockout; Mitochondria; Pyruvic Acid; Seizure; Seizures - genetics; Sialyltransferases - genetics; Sialyltransferases - metabolism; Glucose metabolism; Mitochondria; Seizure; Ganglioside; GM3
• ISSN: 1096-7192; 1096-7206
• DOI: 10.1016/j.ymgme.2022.10.006

GM3 synthase (GM3S) deficiency is a rare neurodevelopmental disorder caused by an inability to synthesize gangliosides, for which there is currently no treatment. Gangliosides are brain-enriched, plasma membrane glycosphingolipids with poorly understood biological functions related to cell adhesion, growth, and receptor-mediated signal transduction. Here, we investigated the effects of GM3S deficiency on metabolism and mitochondrial function in a mouse model. By indirect calorimetry, GM3S knockout mice exhibited increased whole-body respiration and an increased reliance upon carbohydrate as an energy source. 18F-FDG PET confirmed higher brain glucose uptake in knockout mice, and GM3S deficient N41 neuronal cells showed higher glucose utilization in vitro. Brain mitochondria from knockout mice respired at a higher rate on Complex I substrates including pyruvate. This appeared to be due to higher expression of pyruvate dehydrogenase (PDH) and lower phosphorylation of PDH, which would favor pyruvate entry into the mitochondrial TCA cycle. Finally, it was observed that blocking glucose metabolism with the glycolysis inhibitor 2-deoxyglucose reduced seizure intensity in GM3S knockout mice following administration of kainate. In conclusion, GM3S deficiency may be associated with a hypermetabolic phenotype that could promote seizure activity.