Host sequences flanking the HIV provirus

• Published in: Nucleic acids research Vol. 18; no. 20; pp. 6045 - 6047
• Main Authors: VINCENT, K. A, YORK-HIGGINS, D, QUIROGA, M, BROWN, P. O
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25.10.1990
• Subjects: AIDS/HIV; Base Sequence; Biological and medical sciences; Cloning, Molecular; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genic rearrangement. Recombination. Transposable element; Genome, Human; HIV-1 - genetics; Human immunodeficiency virus; Humans; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Oligonucleotide Probes; Plasmids; Polymerase Chain Reaction; Proviruses - genetics; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Sequence Homology, Nucleic Acid; Chromosomal aberration; Integration; Nucleotide sequence; HIV-1 virus; Chromosome insertion; Repeated sequence; Retroviridae; Virus; Polymerase chain reaction; DNA; Abnormal chromosome; Lentivirinae; Human immunodeficiency virus
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/18.20.6045

A conserved property of retroviral proviruses is the presence of a direct repeat in the host DNA immediately flanking the viral sequence; each virus generates a repeat with a characteristic length. By sequencing the viral/host DNA junctions from five HIV-1 proviral clones, we have confirmed that integration of HIV results in the generation of a five basepair direct repeat. A target sequence in uninfected host DNA was analyzed to establish that the five basepair sequence flanking the provirus was present only once prior to integration. Of the five proviruses examined, two were found to have integrated in known repetitive sequence elements of the human genome; one in a Line-1 element and a second in satellite DNA.