The Differential Growth Inhibition of Phytophthora spp. Caused by the Rare Sugar Tagatose Is Associated With Species-Specific Metabolic and Transcriptional Changes
Tagatose is a rare sugar with no negative impacts on human health and selective inhibitory effects on plant-associated microorganisms. Tagatose inhibited mycelial growth and negatively affected mitochondrial processes in Phytophthora infestans , but not in Phytophthora cinnamomi . The aim of this st...
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Published in | Frontiers in microbiology Vol. 12; p. 711545 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media
07.07.2021
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Tagatose is a rare sugar with no negative impacts on human health and selective inhibitory effects on plant-associated microorganisms. Tagatose inhibited mycelial growth and negatively affected mitochondrial processes in
Phytophthora infestans
, but not in
Phytophthora cinnamomi
. The aim of this study was to elucidate metabolic changes and transcriptional reprogramming activated by
P. infestans
and
P. cinnamomi
in response to tagatose, in order to clarify the differential inhibitory mechanisms of tagatose and the species-specific reactions to this rare sugar.
Phytophthora infestans
and
P. cinnamomi
activated distinct metabolic and transcriptional changes in response to the rare sugar. Tagatose negatively affected mycelial growth, sugar content and amino acid content in
P. infestans
with a severe transcriptional reprogramming that included the downregulation of genes involved in transport, sugar metabolism, signal transduction, and growth-related process. Conversely, tagatose incubation upregulated genes related to transport, energy metabolism, sugar metabolism and oxidative stress in
P. cinnamomi
with no negative effects on mycelial growth, sugar content and amino acid content. Differential inhibitory effects of tagatose on
Phytophthora
spp. were associated with an attempted reaction of
P. infestans
, which was not sufficient to attenuate the negative impacts of the rare sugar and with an efficient response of
P. cinnamomi
with the reprogramming of multiple metabolic processes, such as genes related to glucose transport, pentose metabolism, tricarboxylic acid cycle, reactive oxygen species detoxification, mitochondrial and alternative respiration processes. Knowledge on the differential response of
Phytophthora
spp. to tagatose represent a step forward in the understanding functional roles of rare sugars. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Microbial Physiology and Metabolism, a section of the journal Frontiers in Microbiology Reviewed by: Maria Victoria Aguilar Pontes, Concordia University, Canada; Sergio Adrian Guerrero, National University of the Littoral, Argentina Edited by: Alberto A. Iglesias, CONICET Coastline Agrobiotechnology Institute (IAL), Argentina |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.711545 |