Identification and characterization of a calcium-binding peptide from salmon bone for the targeted inhibition of α-amylase in digestion

• Published in: Food Chemistry: X Vol. 22; p. 101352
• Main Authors: Xu, Zhe, Han, Shiying, Cui, Na, Liu, Hanxiong, Yan, Xu, Chen, Hongrui, Wu, Jianping, Tan, Zhijian, Du, Ming, Li, Tingting
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 30.06.2024; Elsevier
• Subjects: Calcium-binding; Peptide; Salmon bone; Targeted inhibition; α-amylase; Targeted inhibition; α-amylase; Salmon bone; Peptide; Calcium-binding
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2024.101352

α-Amylase, essential for carbohydrate digestion, relies on calcium (Ca) for its structural integrity and enzymatic activity. This study explored the inhibitory effect of salmon bone peptides on α-amylase activity through their interaction with the enzyme's Ca-binding sites. Among the various salmon bone hydrolysates, salmon bone trypsin hydrolysate (SBTH) exhibited the highest α-amylase inhibition. The peptide IEELEEELEAER (PIE), with a sequence of Ile-Glu-Glu-Leu-Glu-Glu-Glu-Glu-Leu-Glu-Ala-Glu-Arg from SBTH, was found to specifically target the Ca-binding sites in α-amylase, interacting with key residues such as Asp206, Trp203, His201, etc. Additionally, cellular experiments using 3 T3-L1 preadipocytes indicated PIE's capability to suppress adipocyte differentiation, and decreases in intracellular triglycerides, total cholesterol, and lipid accumulation. In vivo studies also showed a significant reduction in weight gain in the group treated with PIE(6.61%)compared with the control group (33.65%). These findings suggest PIE is an effective α-amylase inhibitor, showing promise for obesity treatment. A calcium-binding peptide from salmon targeted inhibition of amylase in digestion. [Display omitted] •Peptide IEELEELEAER(PIE) should target the inhibition of α-amylase activity.•The site of calcium binding action of PIE in α-amylase by Discovery Studio.•PIE should inhibit the differentiation of 3 T3-L1 and control body weight of mice.