Sensitive determination of aldehyde metabolites in exhaled breath condensate using capillary electrophoresis with laser-induced fluorescence detection

• Published in: Analytical and bioanalytical chemistry Vol. 410; no. 27; pp. 7203 - 7210
• Main Authors: Wang, Tingting, Luo, Dan, Chen, Zheyan, Qu, Yining, Ma, Xiuhua, Ye, Jiannong, Chu, Qingcui, Huang, Dongping
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2018; Springer; Springer Nature B.V
• Subjects: Additives; Aldehydes; Analytical Chemistry; Biochemistry; Biomarkers; Capillary electrophoresis; Capillary zone; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Composition; Condensates; Condensed matter; Electrophoresis; Fluorescein; Food Science; Laboratory Medicine; Laser induced fluorescence; Low molecular weights; Measurement; Metabolism; Metabolites; Methods; Molecular weight; Monitoring/Environmental Analysis; Oxidative metabolism; Oxidative stress; Reagents; Research Paper; Respiration; Smoking; Oxidative stress markers; Noninvasive biofluids; Exhaled breath condensate; Aldehyde metabolites; Capillary electrophoresis with laser-induced fluorescence detection
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-018-1327-9

A novel capillary electrophoresis with laser-induced fluorescence detection method has been developed for the analysis of aldehyde metabolism biomarkers for oxidative stress in exhaled breath condensate (EBC), and fluorescein 5-thiosemicarbazide was used as a derivatization reagent. In a simple capillary zone electrophoresis mode, ten low molecular weight aldehydes (LMWAs) could be well separated within 30 min. The reaction efficiency was doubled by increasing sample solution pH and magnetic stirring, and the LODs of this method reached 0.16–3.4 nM (S/ N  = 3). Acceptable recoveries (82.1–115%) were obtained for EBC samples, and the RSD data were within 7.9%. This developed method has been applied for the analyses of EBC samples and evaluation of the correlation between smoking and the contents of aldehyde metabolites in EBC. Due to no need of buffer additives and sample preconcentration, this proposed method may provide an appealing alternative for the trace analyses of LMWAs in noninvasive biofluids. Graphical abstract ᅟ