Genetic dissection of quantitative trait loci for grain size and weight by high-resolution genetic mapping in bread wheat (Triticum aestivum L.)
Key message Six major QTLs for wheat grain size and weight were identified on chromosomes 4A, 4B, 5A and 6A across multiple environments, and were validated in different genetic backgrounds. Grain size and weight are crucial components of wheat yield. Dissection of their genetic control is thus esse...
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Published in | Theoretical and applied genetics Vol. 135; no. 1; pp. 257 - 271 |
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Main Authors | , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
2022
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
Six major QTLs for wheat grain size and weight were identified on chromosomes 4A, 4B, 5A and 6A across multiple environments, and were validated in different genetic backgrounds.
Grain size and weight are crucial components of wheat yield. Dissection of their genetic control is thus essential for the improvement of yield potential in wheat breeding. We used a doubled haploid (DH) population to detect quantitative trait loci (QTLs) for grain width (GW), grain length (GL), and thousand grain weight (TGW) in five environments. Six major QTLs,
QGw.cib-4B.2
,
QGl.cib-4A
,
QGl.cib-5A.1
,
QGl.cib-6A
,
QTgw.cib-4B
, and
QTgw.cib-5A
, were consistently identified in at least three individual environments and in best linear unbiased prediction (BLUP) datasets, and explained 5.65–34.06% of phenotypic variation.
QGw.cib-4B.2
,
QTgw.cib-4B
,
QGl.cib-5A.1
and
QGl.cib-6A
had no effect on grain number per spike (GNS). In addition to
QGl.cib-4A
, the other major QTLs were further validated by using Kompetitive Allele Specific PCR (KASP) markers in different genetic backgrounds. Moreover, significant interactions between the three major GL QTLs and two major TGW QTLs were observed. Comparison analysis showed that
QGl.cib-5A.1
and
QGl.cib-6A
are likely new loci. Notably,
QGw.cib-4B.2
and
QTgw.cib-4B
were co-located on chromosome 4B and improved TGW by increasing only GW, unlike nearby or overlapped loci reported previously. Three genes associated with grain development within the
QGw.cib-4B.2
/
QTgw.cib-4B
interval were identified by searches on sequence similarity, spatial expression patterns, and orthologs. The major QTLs and KASP markers reported here will be useful for elucidating the genetic architecture of grain size and weight and for developing new wheat cultivars with high and stable yield. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-021-03964-2 |