Effect of Moringa oleifera Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia ( Oreochromis niloticus ) against Streptococcus agalactiae Biotype 2

• Published in: Animals (Basel) Vol. 14; no. 6; p. 953
• Main Authors: Kamble, Manoj Tukaram, Gallardo, Wenresti, Salin, Krishna Rugmini, Pumpuang, Suntree, Chavan, Balasaheb Ramdas, Bhujel, Ram Chandra, Medhe, Seema Vijay, Kettawan, Aikkarach, Thompson, Kim D, Pirarat, Nopadon
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.03.2024; MDPI
• Subjects: Antimicrobial agents; Aquaculture; Flavonoids; growth performance; Hematology; immune response; Moringa oleifera; Nile tilapia; Streptococcus agalactiae Biotype 2; Tilapia; Thailand; United States > US; Germany; growth performance; hematology; Moringa oleifera; Nile tilapia; immune response; Streptococcus agalactiae Biotype 2
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani14060953

The present study aimed to investigate the effects of leaf (MLE) extract-supplemented diets on the growth, feed utilization, hematology, innate immune response, and disease resistance of Nile tilapia against Biotype 2. Four hundred and fifty Nile tilapia (32.61 ± 0.2 g/fish) were randomly allocated into fifteen tanks (30 fish/tank). Different concentrations of MLE at 0%, 0.5%, 1%, 1.5%, and 2% were fed to the Nile tilapia for 30 days, and the growth, feed utilization, hematology, and innate immune response of the Nile tilapia were determined. After the feeding trial, the Nile tilapia were challenged with a Biotype 2 infection, and the relative percentage of survival (RPS) was determined. Results revealed the presence of quercetin, kaempferol, and -coumaric acid in the MLE extract, exhibiting stronger antimicrobial activity against Biotype 2. The diets supplemented with the MLE-0.5 group showed a significantly higher growth, feed utilization, hematology, and innate immune response in the Nile tilapia compared to the control and other MLE groups. Additionally, the MLE-0.5 group exhibited a significantly higher RPS of the Nile tilapia against Biotype 2. Therefore, MLE-0.5 can be employed as an alternative feed supplement in sustainable Nile tilapia farming to protect against Biotype 2.