Molecular Typing of Candida glabrata

• Published in: Mycopathologia (1975) Vol. 185; no. 5; pp. 755 - 764
• Main Authors: Gabaldón, Toni, Gómez-Molero, Emilia, Bader, Oliver
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2020; Springer; Springer Nature B.V
• Subjects: Animal behavior; Antifungal agents; Antifungal Agents - pharmacology; Biofilms; Biomedical and Life Sciences; Candida glabrata; Candida glabrata - genetics; Candida glabrata - isolation & purification; Candidiasis - drug therapy; Catheters; DNA Probes; DNA sequencing; Drug resistance; Drug Resistance, Fungal - genetics; Eukaryotic Microbiology; Fungi; Genes, Fungal; Genetic polymorphisms; Genetically modified organisms; Genome, Fungal; Genomes; Genomics; Genotyping Techniques - methods; Humans; Life Sciences; Medical Microbiology; Microbial Ecology; Microbiology; Microsatellite Repeats; Multilocus Sequence Typing; Mycological Typing Techniques - methods; Nucleotide sequence; Nucleotide sequencing; Pathogenicity; Pathogens; Plant Sciences; Population; Population genetics; Population structure; Review; Scientific imaging; Virulence; Whole Genome Sequencing; Microsatellite typing (MLP); pubmlst.org; Multi-locus sequence typing (MLST); Whole-genome sequencing (WGS); Candida glabrata
• ISSN: 0301-486X; 1573-0832; 1573-0832
• DOI: 10.1007/s11046-019-00388-x

The yeast Candida glabrata has emerged, second only to Candida albicans , to be one of the most frequently isolated fungi in clinical specimen from human. Its frequent resistance towards azole antifungal drugs and the high capacity to form biofilms on indwelling catheters of individual isolates render it an often difficult to treat pathogen. Hence, there is a notably increasing scientific and clinical interest in this species. This has led to the development of a variety of molecular tools for genetic modification, strain collections, and last but not least different approaches to analyse the population structure among isolates of different geographical and clinical contexts. Often, these are used to study correlations (or the absence thereof) with different pathogenicity, virulence, or drug resistance traits. Three molecular methods have been used to type within the C. glabrata population on a genetic level by multiple studies: multi-locus sequence typing, microsatellite length polymorphisms, and clustering of whole-genome sequencing data, and these are subject of this review.