Characterization of an Injury Induced Population of Muscle-Derived Stem Cell-Like Cells

• Published in: Scientific reports Vol. 5; no. 1; p. 17355
• Main Authors: Vojnits, Kinga, Pan, HaiYing, Mu, Xiaodong, Li, Yong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.11.2015; Nature Publishing Group
• Subjects: 13/100; 13/106; 13/51; 14/63; 38; 38/77; 42/35; 631/532/2439; 64/60; 692/308/2171; 82/1; Animals; Biomarkers - metabolism; Blastocyst - cytology; Blastocyst - metabolism; Cell Differentiation; Cell Movement; Cellular Reprogramming; Embryo, Mammalian; Embryoid Bodies - cytology; Embryoid Bodies - metabolism; Gene Expression; Germ Layers - cytology; Germ Layers - metabolism; Humanities and Social Sciences; Lewis X Antigen - genetics; Lewis X Antigen - metabolism; Mice; Mice, Inbred C57BL; Microinjections; MSX1 Transcription Factor - genetics; MSX1 Transcription Factor - metabolism; multidisciplinary; Muscle, Skeletal - cytology; Muscle, Skeletal - injuries; Muscle, Skeletal - metabolism; Myoblasts, Skeletal - cytology; Myoblasts, Skeletal - metabolism; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Receptors, CXCR4 - genetics; Receptors, CXCR4 - metabolism; Science; SOXB1 Transcription Factors - genetics; SOXB1 Transcription Factors - metabolism; Teratoma - metabolism; Teratoma - pathology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep17355

We recently discovered a novel population of stem cells from the injured murine skeletal muscle. These injury induced muscle-derived stem cell-like cells (iMuSCs) are partially reprogrammed from differentiated myogenic cells and display a pluripotent-like state. The iMuSCs exhibit stem cell properties including the ability to differentiate into multiple lineages, such as neurogenic and myogenic differentiations; they also display a superior migration capacity that demonstrating a strong ability of muscle engraftment in vivo . IMuSCs express several pluripotent and myogenic stem cell markers; have the capability to form embryoid bodies and teratomas and can differentiate into all three germ layers. Moreover, blastocyst microinjection showed that the iMuSCs contributed to chimeric embryos but could not complete germline transmission. Our results indicate that the iMuSCs are in a partially reprogrammed state of pluripotency, which are generated by the microenvironment of injured skeletal muscle.