Effects of air humidity and soil moisture on secondary metabolites in the leaves and roots of Betula pendula of different competitive status

• Published in: Oecologia Vol. 202; no. 2; pp. 193 - 210
• Main Authors: Kharel, Bikash, Rusalepp, Linda, Bhattarai, Biplabi, Kaasik, Ants, Kupper, Priit, Lutter, Reimo, Mänd, Pille, Rohula-Okunev, Gristin, Rosenvald, Katrin, Tullus, Arvo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2023; Springer; Springer Nature B.V
• Subjects: Abiotic factors; Air; Analysis; Betula pendula; Biomedical and Life Sciences; Canopies; Canopy; Carbon; Chromatography; Climate change; Competition; Dihydroxybenzoic acid; Ecology; Flavonoids; Global temperature changes; Glucosides; Glycosides; Herbivory; High performance liquid chromatography; Highlighted Student Research; HPLC; Humidity; Hydrology/Water Resources; Jasmonic acid; Leaves; Life Sciences; Liquid chromatography; Metabolites; Moisture effects; Plant cover; Plant metabolites; Plant Sciences; Quadrupoles; Relative humidity; Secondary metabolites; Sesquiterpenoids; Soil; Soil conditions; Soil moisture; Stresses; Tradeoffs; Trees; Climate change; Environmental humidity; Growth-defence; Phenolics; Silver birch
• ISSN: 0029-8549; 1432-1939
• DOI: 10.1007/s00442-023-05388-9

Plant secondary metabolites (PSMs) defend plants against abiotic stresses, including those caused by climate change and against biotic stresses, such as herbivory and competition. There is a trade-off between allocating available carbon to growth and defence in stressful environments. However, our knowledge about trade-off is limited, especially when abiotic and biotic stresses co-occur. We aimed to understand the combined effect of increasing precipitation and humidity, the tree's competitive status, and canopy position on leaf secondary metabolites (LSMs) and fine root secondary metabolites (RSMs) in Betula pendula . We sampled 8-year-old B. pendula trees growing in the free air humidity manipulation (FAHM) experimental site, where treatments included elevated relative air humidity and elevated soil moisture. A high-performance liquid chromatography–quadrupole-time of flight mass spectrometer (HPLC–qTOF-MS) was used to analyse secondary metabolites. Our results showed accumulation of LSM depends on the canopy position and competitive status. Flavonoids (FLA), dihydroxybenzoic acids (HBA), jasmonates (JA) and terpene glucosides (TG) were higher in the upper canopy, and FLA, monoaryl compounds (MAR) and sesquiterpenoids (ST) were higher in dominant trees. The FAHM treatments had a more distinct effect on RSM than on LSM. The RSMs were lower in elevated air humidity and soil moisture conditions than in control conditions. The RSM content depended on the competitive status and was higher in suppressed trees. Our study suggests that young B. pendula will allocate similar amounts of carbon to constitutive chemical leaf defence, but a lower amount to root defence (per fine root biomass) under higher humidity.