Green Synthesis of Zinc Oxide Nanoparticles Using an Aqueous Extract of Punica granatum for Antimicrobial and Catalytic Activity

• Published in: Journal of functional biomaterials Vol. 14; no. 4; p. 205
• Main Authors: Fouda, Amr, Saied, Ebrahim, Eid, Ahmed M, Kouadri, Fayza, Alemam, Ahmed M, Hamza, Mohammed F, Alharbi, Maha, Elkelish, Amr, Hassan, Saad El-Din
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.04.2023; MDPI
• Subjects: Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Bacteria; Catalysts; Catalytic activity; Crystal structure; Crystallography; Data analysis; Degradation; Drug resistance; Dyes; Effectiveness; Efficiency; Electron microscopy; Fourier transforms; Free radicals; Gram-negative bacteria; Gram-positive bacteria; Hypotheses; Light emission; Metal oxides; Methylene blue; Microorganisms; Microscopy; Minimum inhibitory concentration; Nanomaterials; Nanoparticles; Organic chemicals; Photocatalysis; plant-based ZnO-NPs; Pollutants; Punica granatum; ROS; Scanning electron microscopy; Textiles; Transmission electron microscopy; Ultraviolet radiation; Ultraviolet spectroscopy; Water pollution; X-ray diffraction; Zinc oxide; Zinc oxides; Cairo Egypt; Egypt; Punica granatum; free radicals; antimicrobial activity; photocatalysis; plant-based ZnO-NPs; ROS
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb14040205

The peel aqueous extract of was utilized to fabricate zinc oxide nanoparticles (ZnO-NPs) as a green approach. The synthesized NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy, which was attached to an energy dispersive X-ray (SEM-EDX). Spherical, well arranged, and crystallographic structures of ZnO-NPs were formed with sizes of 10-45 nm. The biological activities of ZnO-NPs, including antimicrobial and catalytic activity for methylene blue dye, were assessed. Data analysis showed that the antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria, as well as unicellular fungi, was observed to occur in a dose-dependent manner, displaying varied inhibition zones and low minimum inhibitory concentration (MIC) values in the ranges of 6.25-12.5 µg mL . The degradation efficacy of methylene blue (MB) using ZnO-NPs is dependent on nano-catalyst concentration, contact time, and incubation condition (UV-light emission). The maximum MB degradation percentages of 93.4 ± 0.2% was attained at 20 µg mL after 210 min in presence of UV-light. Data analysis showed that there is no significant difference between the degradation percentages after 210, 1440, and 1800 min. Moreover, the nano-catalyst showed high stability and efficacy to degrade MB for five cycles with decreasing values of 4%. Overall, -based ZnO-NPs are promising tools to inhibit the growth of pathogenic microbes and degradation of MB in the presence of UV-light emission.