Lignin degradation by peroxidase in organic media: a reassessment

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 84; no. 22; pp. 7925 - 7927
• Main Authors: Lewis, N.G, Razal, R.A, Yamamoto, E
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.11.1987; National Acad Sciences
• Subjects: Biochemistry; Biological and medical sciences; Biological markers; Biological Sciences: Biochemistry; Biotechnology; DEGRADATION; Depolymerization; DETERIORO; Dioxanes; Elution; Enzyme activity; Enzyme engineering; Enzymes; EXTRACTOS VEGETALES; EXTRAIT D'ORIGINE VEGETALE; Fundamental and applied biological sciences. Psychology; Lignin; LIGNINAS; ligninase; LIGNINE; LIGNINS; Materials; Methods. Procedures. Technologies; Miscellaneous; Molecular weight; PEROXIDASAS; PEROXIDASES; PEROXYDASE; PLANT EXTRACTS; Biodegradation; Lignin; Organic solvent; Peroxidase; In vitro
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.84.22.7925

The effect of horseradish peroxidase/H2O2 in organic medium (dioxane/aqueous buffer, pH 5, 95:5) on the depolymerization of synthetic (dehydrogenatively polymerized) lignin was reinvestigated. In contrast to previous claims [Dordick, J. S., Marletta, M. A. & Klibanov, A. M. (1986) Proc. Natl. Acad. Sci. USA 83, 6255-6257], our results demonstrated that vigorous depolymerization of this substrate did not occur. Further, during this treatment ferulic acid was not a significant biodegradation product.