Gene transfer into cardiac myocytes

Traditional methods for DNA transfection are often inefficient and toxic for terminally differentiated cells, such as cardiac myocytes. Vector-based gene transfer is an efficient approach for introducing exogenous cDNA into these types of primary cell cultures. In this chapter, separate protocols fo...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1299; p. 177
Main Authors Lang, Sarah E, Westfall, Margaret V
Format Journal Article
LanguageEnglish
Published United States 01.01.2015
Subjects
Adenoviridae - genetics
Animals
Cell Culture Techniques
Cell Separation - methods
Gene Transfer Techniques
Genetic Vectors - genetics
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
Rats
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Summary:Traditional methods for DNA transfection are often inefficient and toxic for terminally differentiated cells, such as cardiac myocytes. Vector-based gene transfer is an efficient approach for introducing exogenous cDNA into these types of primary cell cultures. In this chapter, separate protocols for adult rat cardiac myocyte isolation and gene transfer with recombinant adenovirus are provided and are routinely utilized for studying the effects of sarcomeric proteins on myofilament function.
ISSN:1940-6029
DOI:10.1007/978-1-4939-2572-8_15