Mapping and characterization of two stem rust resistance genes derived from cultivated emmer wheat accession PI 193883
Key message Two stem rust resistance genes identified on chromosome arms 2BL and 6AL of the cultivated emmer wheat accession PI 193883 can be used for protecting modern varieties against Ug99 strains. The wheat research community consistently strives to identify new genes that confer resistance to s...
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Published in | Theoretical and applied genetics Vol. 132; no. 11; pp. 3177 - 3189 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.11.2019
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
Two stem rust resistance genes identified on chromosome arms 2BL and 6AL of the cultivated emmer wheat accession PI 193883 can be used for protecting modern varieties against Ug99 strains.
The wheat research community consistently strives to identify new genes that confer resistance to stem rust caused by the fungal pathogen
Puccinia graminis
f. sp
. tritici
Eriks & E. Henn (
Pgt
). In the current study, our objective was to identify and genetically characterize the stem rust resistance derived from the cultivated emmer accession PI 193883. A recombinant inbred line population developed from a cross between the susceptible durum wheat line Rusty and PI 193883 was genotyped and evaluated for reaction to
Pgt
races TTKSK, TRTTF, and TMLKC. Two QTLs conferring resistance were identified on chromosome arms 2BL (
QSr.fcu
-
2B
) and 6AL (
QSr.fcu
-
6A
). The stem rust resistance gene (
Sr883
-
2B
) underlying
QSr.fcu
-
2B
was recessive, and based on its physical location it is located proximal to the
Sr9
region.
QSr.fcu
-
6A
was located in the
Sr13
region, but PI 193883 is known to carry the susceptible haplotype S4 for
Sr13
, indicating that the gene underlying
QSr.fcu
-
6A
(
Sr883
-
6A
) is likely a new allele of
Sr13
or a gene residing close to
Sr13
. Three IWGSC scaffold-based simple sequence repeat (SSR) and two SNP-based semi-thermal asymmetric reverse PCR (STARP) markers were developed for the
Sr883
-
2B
region, and one STARP marker was developed for
Sr883
-
6A
.
Sr883
-
2B
was epistatic to
Sr883
-
6A
for reaction to TTKSK and TRTTF, and the two genes had additive effects for TMLKC. These two genes and the markers developed in this research provide additional resources and tools for the improvement in stem rust resistance in durum and common wheat breeding programs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/s00122-019-03417-x |