PD-L2 is expressed on activated human T cells and regulates their function

• Published in: Molecular immunology Vol. 48; no. 15-16; pp. 2214 - 2219
• Main Authors: Messal, Nassima, Serriari, Nacer-Eddine, Pastor, Sonia, Nunès, Jacques A., Olive, Daniel
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2011; Elsevier
• Subjects: Animals; Antigens, CD80; B7-1 Antigen - biosynthesis; B7-1 Antigen - immunology; Cell activation; Cell Separation; Costimulatory molecules; Cytokines; Cytokines - biosynthesis; Cytokines - immunology; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Humans; Immunology; Life Sciences; Lymphocyte Activation; Lymphocyte Activation - immunology; Mice; Mice, Inbred BALB C; PD-L2; Programmed Cell Death 1 Ligand 2 Protein; T cells; T-Lymphocytes; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; APCs; TcR; Cell activation; Costimulatory molecules; PD-L; PD-L2; T cells; PD-1; mAb; cell activation; costimulatory molecules
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/j.molimm.2011.06.436

• The cosignaling molecule, PD-L2 is expressed on human activated T cell subsets. • The PD-L2 triggering down-regulates (TCR+CD28)-induced cytokine production on human T cells. • A direct targeting of PD-L2 on T cells blocks T cell proliferation. T-cell activation and proliferation are regulated by cosignaling adhesion molecules involved in positive or negative signals. Programmed death (PD)-1 is one of immune inhibitory molecules that is expressed in activated T cells and is a promising target for immunotherapy. Both PD-1 ligands, PD-L1 and PD-L2 are expressed on antigen presenting cells (APCs) involved in the dialogue between a T cell and an APC. Here, we analysed the expression of these ligands, especially for PD-L2, on T cells. PD-L2 appears to be expressed on activated CD4 and CD8T cell subsets. Moreover, as PD-1 molecule, PD-L2 engagement at the surface of T cells is able to down-modulate cytokine production and cell proliferation. These observations indicate that PD-L2 is expressed following activation and is involved in the regulation of T cell function, highlighting the level of complexity in the T cell cosignaling network.