Rational design of memory in eukaryotic cells

The ability to logically engineer novel cellular functions promises a deeper understanding of biological systems. Here we demonstrate the rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback. We built a set of transcriptional activators and quanti...

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Bibliographic Details
Published inGenes & development Vol. 21; no. 18; pp. 2271 - 2276
Main Authors Ajo-Franklin, Caroline M, Drubin, David A, Eskin, Julian A, Gee, Elaine P S, Landgraf, Dirk, Phillips, Ira, Silver, Pamela A
Format Journal Article
LanguageEnglish
Published United States Cold Spring Harbor Laboratory Press 15.09.2007
Subjects
Eukaryotic Cells - metabolism
Eukaryotic Cells - physiology
Feedback, Physiological - physiology
Gene Regulatory Networks - physiology
Genes, Reporter
Organisms, Genetically Modified
Research Communication
Research Design
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Trans-Activators - physiology
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Summary:The ability to logically engineer novel cellular functions promises a deeper understanding of biological systems. Here we demonstrate the rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback. We built a set of transcriptional activators and quantitatively characterized their effects on gene expression in living cells. Modeling in conjunction with the quantitative characterization of the activator-promoter pairs accurately predicts the behavior of the memory network. This study demonstrates the power of taking advantage of components with measured quantitative parameters to specify eukaryotic regulatory networks with desired properties.
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Present addresses: Lawrence Berkeley National Laboratories, 1 Cyclotron Rd., MS 67R5110, Berkeley, CA 94720, USA
These authors contributed equally to this work.
Vanderbilt Medical School, 201 Light Hall, Nashville, TN 37232, USA.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1586107