Amperometric DNA-Peroxidase Sensor for the Detection of Pharmaceutical Preparations

• Published in: Sensors (Basel, Switzerland) Vol. 5; no. 6; pp. 364 - 376
• Main Authors: Evtugyn, G. A., Goldfarb, O. E., Budnikov, H. C., Ivanov, A. N., Vinter, V. G.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 14.11.2005; Molecular Diversity Preservation International (MDPI)
• Subjects: affinity biosensor; anthracycline determination; Antibodies; Biosensors; Carbon; Cytochrome; DNA sensor; Electrodes; Electrons; Enzymes; horseradish peroxidase; Hybridization; Oxidation; Pharmaceuticals; Phosphatase; Sensors; sulfonamide determination; Russia
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s5060364

Novel DNA-sensor with enzymatic amplification of the signal has beendeveloped on the base of glassy carbon electrode modified with ds-DNA and horseradishperoxidase (HRP). Phenothiazine dyes Methylene Blue and Methylene Green were used aselectrochemical markers for the detection of sulfonamide and anthracycline preparationsable to interact with DNA. The biosensor signal related to HRP oxidation of the markersdepends on the relation between their bonded and readily oxidized forms which depends onthe nature and concentration of pharmaceuticals. Sulfonamides diminish surfaceconcentration of MB accessible for HRP reaction whereas anthracyclines releaseintercalated marker and increase the signal. The DNA-HRP sensor developed makes itpossible to detect down to 0.002 nmol L-1 of sulfamethoxazole, 0.1 nmol L-1 of sulfadiazine,0.01 nmol L-1 of sulfamethazine, 0.1 nmol L-1 of sulfaguanine, 0.05 μmol L-1 of rubomycinand 0.08 μmol L-1 of doxorubicin.