The 3-Year Effect of the Mediterranean Diet Intervention on Inflammatory Biomarkers Related to Cardiovascular Disease

• Published in: Biomedicines Vol. 9; no. 8; p. 862
• Main Authors: Urpi-Sarda, Mireia, Casas, Rosa, Sacanella, Emilio, Corella, Dolores, Andrés-Lacueva, Cristina, Llorach, Rafael, Garrabou, Gloria, Cardellach, Francesc, Sala-Vila, Aleix, Ros, Emilio, Ruiz-Canela, Miguel, Fitó, Montserrat, Salas-Salvadó, Jordi, Estruch, Ramon
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22.07.2021; MDPI
• Subjects: Anti-inflammatory agents; Arteriosclerosis; Atherosclerosis; Biomarkers; Blood pressure; Body mass index; Cardiovascular disease; Cardiovascular diseases; Chemokines; Cholesterol; CXCR2 protein; CXCR3 protein; Cytokines; Diet; dietary pattern; Drug use; Fatty acids; Gene expression; IL-1β; Inflammation; Interleukin 6; Interleukin 8; Intervention; Low fat diet; Monocyte chemoattractant protein 1; Nutrient deficiency; nutrition; Nuts; Olive oil; Plasma; Plasma levels; Polymerase chain reaction; Proteins; RANTES; Software; Tumor necrosis factor-α; γ-Interferon; United States > US
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines9080862

The intervention with the Mediterranean diet (MD) pattern has evidenced short-term anti-inflammatory effects, but little is known about its long-term anti-inflammatory properties at molecular level. This study aims to investigate the 3-year effect of MD interventions compared to low-fat diet (LFD) on changes on inflammatory biomarkers related to atherosclerosis in a free-living population with a high-risk of cardiovascular disease (CD). Participants (n = 285) in the PREDIMED trial were randomly assigned into three intervention groups: MD with extra-virgin olive oil (EVOO) or MD-Nuts, and a LFD. Fourteen plasma inflammatory biomarkers were determined by Luminex assays. An additional pilot study of gene expression (GE) was determined by RT-PCR in 35 participants. After 3 years, both MDs showed a significant reduction in the plasma levels of IL-1β, IL-6, IL-8, TNF-α, IFN-γ, hs-CRP, MCP-1, MIP-1β, RANTES, and ENA78 (p < 0.05; all). The decreased levels of IL-1β, IL-6, IL-8, and TNF-α after MD significantly differed from those in the LFD (p < 0.05). No significant changes were observed at the gene level after MD interventions, however, the GE of CXCR2 and CXCR3 tended to increase in the control LFD group (p = 0.09). This study supports the implementation of MD as a healthy long-term dietary pattern in the prevention of CD in populations at high cardiovascular risk.