A lectin S-domain receptor kinase mediates lipopolysaccharide sensing in Arabidopsis thaliana
Plants have a vast array of pattern-recognition receptor to detect microbial ligands, but how they sense lipopolysaccharide has remained unknown. Ranf et al. identify the receptor-like kinase SD1-29 as a de facto pattern-recognition receptor for lipopolysaccharide in Arabidopsis . The sensing of mic...
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Published in | Nature immunology Vol. 16; no. 4; pp. 426 - 433 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.04.2015
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Plants have a vast array of pattern-recognition receptor to detect microbial ligands, but how they sense lipopolysaccharide has remained unknown. Ranf
et al.
identify the receptor-like kinase SD1-29 as a
de facto
pattern-recognition receptor for lipopolysaccharide in
Arabidopsis
.
The sensing of microbe-associated molecular patterns (MAMPs) triggers innate immunity in animals and plants. Lipopolysaccharide (LPS) from Gram-negative bacteria is a potent MAMP for mammals, with the lipid A moiety activating proinflammatory responses via Toll-like receptor 4 (TLR4). Here we found that the plant
Arabidopsis thaliana
specifically sensed LPS of
Pseudomonas
and
Xanthomonas
. We isolated LPS-insensitive mutants defective in the bulb-type lectin S-domain-1 receptor–like kinase LORE (SD1-29), which were hypersusceptible to infection with
Pseudomonas syringae
. Targeted chemical degradation of LPS from
Pseudomonas
species suggested that LORE detected mainly the lipid A moiety of LPS. LORE conferred sensitivity to LPS onto tobacco after transient expression, which demonstrated a key function in LPS sensing and indicated the possibility of engineering resistance to bacteria in crop species. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1529-2908 1529-2916 |
DOI: | 10.1038/ni.3124 |