MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

• Published in: Journal of cellular and molecular medicine Vol. 22; no. 4; pp. 2357 - 2367
• Main Authors: Diao, Yuling, Jin, Baozhe, Huang, Liyong, Zhou, Wenke
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.04.2018; John Wiley and Sons Inc
• Subjects: Apoptosis; Assaying; Cancer therapies; Cell adhesion & migration; Cell culture; Cell cycle; Cell migration; Cell proliferation; Cholecystokinin; Experiments; Gastric cancer; Gene expression; Glioma; Glioma cells; Invasiveness; Laboratory animals; Lung cancer; Medical prognosis; Medical research; Metastasis; MicroRNAs; miRNA; miR‐129‐5p; Nervous system; Original; Stem cells; TGIF2; Tissues; Tumorigenesis; Tumors; Wound healing; Xenografts; United States > US; China; Japan; glioma; TGIF2; miR-129-5p
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.13529

This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cytometer, transwell assay and wound‐healing assay were employed to detect cell proliferation, apoptosis and cycle, invasiveness and migration, respectively. Dual‐luciferase reporting assay was performed to confirm the targeted relationship between miR‐129‐5p and TGIF2. The effects of TGIF2 expression on cell biological functions were also investigated using the indicated methods. Tumour xenograft was applied to explore the impact of miR‐129‐5p on tumorigenesis in vivo. MiR‐129‐5p expression was down‐regulated in both glioma tissues and glioma cells, while TGIF2 expression was aberrantly higher than normal level. Dual‐luciferase reporter assay validated the targeting relation between miR‐129‐5p and TGIF2. Overexpression of miR‐129‐5p or down‐regulation of TGIF2 inhibited the proliferation, invasion and migration capacity of glioma cells U87 and U251, and meanwhile blocked the cell cycle as well as induced cell apoptosis. MiR‐129‐5p overexpression repressed the tumour development in vivo. MiR‐129‐5p and TGIF2 had opposite biological functions in glioma cells. MiR‐129‐5p could inhibit glioma cell progression by targeting TGIF2, shining light for the development of target treatment for glioma.