Localization of BDNF expression in the developing brain of zebrafish

• Published in: Journal of anatomy Vol. 224; no. 5; pp. 564 - 574
• Main Authors: De Felice, E., Porreca, I., Alleva, E., De Girolamo, P., Ambrosino, C., Ciriaco, E., Germanà, A., Sordino, P.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.05.2014; Blackwell Science Inc
• Subjects: Animals; Brain; Brain - embryology; Brain - growth & development; Brain - metabolism; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - genetics; Brain-Derived Neurotrophic Factor - metabolism; central nervous system; Danio rerio; development; Freshwater; Gene Expression Regulation, Developmental; In Situ Hybridization; Neurons - metabolism; real‐time quantitative polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; whole‐mount in situ hybridization; Zebrafish; real-time quantitative polymerase chain reaction; development; brain-derived neurotrophic factor; zebrafish; whole-mount in situ hybridization; central nervous system
• ISSN: 0021-8782; 1469-7580
• DOI: 10.1111/joa.12168

The brain‐derived neurotrophic factor (BDNF) gene is expressed in differentiating and post‐mitotic neurons of the zebrafish embryo, where it has been implicated in Huntington's disease. Little is known, however, about the full complement of neuronal cell types that express BDNF in this important vertebrate model. Here, we further explored the transcriptional profiles during the first week of development using real‐time quantitative polymerase chain reaction (RT‐qPCR) and whole‐mount in situ hybridization (WISH). RT‐qPCR results revealed a high level of maternal contribution followed by a steady increase of zygotic transcription, consistent with the notion of a prominent role of BDNF in neuronal maturation and maintenance. Based on WISH, we demonstrate for the first time that BDNF expression in the developing brain of zebrafish is structure specific. Anatomical criteria and co‐staining with genetic markers (shh, pax2a, emx1, krox20, lhx2b and lhx9) visualized major topological domains of BDNF‐positive cells in the pallium, hypothalamus, posterior tuberculum and optic tectum. Moreover, the relative timing of BDNF transcription in the eye and tectum may illustrate a mechanism for coordinated development of the retinotectal system. Taken together, our results are compatible with a local delivery and early role of BDNF in the developing brain of zebrafish, adding basic knowledge to the study of neurotrophin functions in neural development and disease.