Reconfigurable Bioinspired Framework Nucleic Acid Nanoplatform Dynamically Manipulated in Living Cells for Subcellular Imaging

In nature, the formation of spider silk fibers begins with dimerizing the pH‐sensitive N‐terminal domains of silk proteins (spidroins) upon lowering pH, and provides a natural masterpiece for programmable assembly. Inspired by the similarity of pH‐dependent dimerization behaviors, introduced here is...

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Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 58; no. 6; pp. 1648 - 1653
Main Authors Peng, Pai, Du, Yi, Zheng, Jiao, Wang, Huihui, Li, Tao
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 04.02.2019
EditionInternational ed. in English
Subjects
Aptamers
Assembly
Biomimetics
Cells (biology)
Deoxyribonucleic acid
Dimerization
DNA
Fibers
Lysosomes
nanostructures
Nucleic acids
pH effects
Proteins
self-assembly
Silk
supramolecular chemistry
self-assembly
nucleic acids
nanostructures
supramolecular chemistry
DNA
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Summary:In nature, the formation of spider silk fibers begins with dimerizing the pH‐sensitive N‐terminal domains of silk proteins (spidroins) upon lowering pH, and provides a natural masterpiece for programmable assembly. Inspired by the similarity of pH‐dependent dimerization behaviors, introduced here is an i‐motif‐guided model to mimic the initial step of spidroin assembly at the subcellular level. A framework nucleic acid (FNA) nanoplatform is designed using two tetrahedral DNA nanostructures (TDNs) with different branched vertexes carrying a bimolecular i‐motif and a split ATP aptamer. Once TDNs enter acidic lysosomes within living cells, they assemble into a heterodimeric architecture, thereby enabling the formation of a larger‐size framework and meanwhile subcellular imaging in response to endogenous ATP, which can be dynamically manipulated by adjusting intracellular pH and ATP levels with external drug stimuli. Itsy bitsy spider: To mimic the self‐assembly manner of spider silk proteins, a reconfigurable bioinspired framework nucleic acid nanoplatform has been designed and is dynamically regulated within living cells for lysosome imaging. The dimerization of the DNA structures is pH‐dependent and they enable subcellular imaging in response to endogenous ATP.
Bibliography:These authors contributed equally to this work.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201811117