GDF‐5 induces epidermal stem cell migration via RhoA‐MMP9 signalling
The migration of epidermal stem cells (EpSCs) is critical for wound re‐epithelization and wound healing. Recently, growth/differentiation factor‐5 (GDF‐5) was discovered to have multiple biological effects on wound healing; however, its role in EpSCs remains unclear. In this work, recombinant mouse...
Saved in:
Published in | Journal of cellular and molecular medicine Vol. 25; no. 4; pp. 1939 - 1948 |
---|---|
Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
John Wiley & Sons, Inc
01.02.2021
John Wiley and Sons Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The migration of epidermal stem cells (EpSCs) is critical for wound re‐epithelization and wound healing. Recently, growth/differentiation factor‐5 (GDF‐5) was discovered to have multiple biological effects on wound healing; however, its role in EpSCs remains unclear. In this work, recombinant mouse GDF‐5 (rmGDF‐5) was found via live imaging in vitro to facilitate the migration of mouse EpSCs in a wound‐scratch model. Western blot and real‐time PCR assays demonstrated that the expression levels of RhoA and matrix metalloproteinase‐9 (MMP9) were correlated with rmGDF‐5 concentration. Furthermore, we found that rmGDF‐5 stimulated mouse EpSC migration in vitro by regulating MMP9 expression at the mRNA and protein levels through the RhoA signalling pathway. Moreover, in a deep partial‐thickness scald mouse model in vivo, GDF‐5 was confirmed to promote EpSC migration and MMP9 expression via RhoA, as evidenced by the tracking of cells labelled with 5‐bromo‐2‐deoxyuridine (BrdU). The current study showed that rmGDF‐5 can promote mouse EpSC migration in vitro and in vivo and that GDF‐5 can trigger the migration of EpSCs via RhoA‐MMP9 signalling. |
---|---|
Bibliography: | This work was supported by grants from the National Natural Science Foundation of China (81701925), the Postdoctoral Science Foundation of China (2018M633760) and Natural Science Foundation Innovation Group Science Foundation of Chongqing (cstc2019jcyj‐cxttX0001). The funders had no roles in the study design, data collection and analysis, decision to publish or preparation of the manuscript. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Funding information Xue Li, Fan Wang and Yuanxin Lan are contributed equally to this work. |
ISSN: | 1582-1838 1582-4934 |
DOI: | 10.1111/jcmm.15925 |