Metabolic diversity in apple germplasm

We analysed metabolic diversity in apples from wild species, elite material and a F₁ population, using liquid chromatography–mass spectrometry (LC‐QTOF‐MS). The evaluated elite material appeared to have strongly reduced levels of phenolic compounds, down to 1% of the concentrations in the investigat...

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Published inPlant breeding Vol. 133; no. 2; pp. 281 - 290
Main Authors Khan, Sabaz A, Tikunov, Yury, Chibon, Pierre‐Yves, Maliepaard, Chris, Beekwilder, Jules, Jacobsen, Evert, Schouten, Henk J, Flachowsky, H
Format Journal Article
LanguageEnglish
Published Berlin Blackwell Publishing Ltd 01.04.2014
Wiley Subscription Services, Inc
Subjects
alleles
apple
apples
Enoyl-CoA hydratase
epicatechin
fruits
Genes
genetic diversity
genome
Germplasm
LC-QTOF-MS
linkage group 16
Malus
malus-sieversii
mass spectrometry
metabolic diversity
Metabolites
mqtl hotspot
mQTL mapping
origin
parents
phenolic compounds
progeny
rosaceae
structural genes
transgressive segregation
x domestica borkh
β-glycols
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Summary:We analysed metabolic diversity in apples from wild species, elite material and a F₁ population, using liquid chromatography–mass spectrometry (LC‐QTOF‐MS). The evaluated elite material appeared to have strongly reduced levels of phenolic compounds, down to 1% of the concentrations in the investigated wild germplasm. In one quarter of the F₁ population, the concentrations of phenolic compounds such as quercetin derivatives, procyanidin, catechin and epicatechin were further significantly reduced, due to accumulation of recessive alleles of putatively leucoanthocyanidin reductase, a structural gene that is located at the top of LG16. In another part of F₁ progeny, putatively glycosylated forms of β‐glycols were up to 50 times more abundant compared to both parents. These metabolites were mapped with high logarithm of odds (LOD) scores at the top of LG8, and progeny that was homozygous recessive for the candidate gene showed the elevated levels. We hypothesize that this was caused by inheritance of non‐functional alleles of enoyl‐CoA hydratase gene. Both examples of transgressive segregation, where some progeny significantly deviated from both parents, were caused by accumulation of recessive alleles.
Bibliography:http://dx.doi.org/10.1111/pbr.12134
PiDON
Higher Education Commission (HEC) of Pakistan
Inova Fruit B.V.
Figure S1. a: Peel; Metabolites having much higher levels in some progeny than in both parents. b: Flesh; Metabolites having much higher levels in some progeny than in both parents. Table S1. Putative metabolites in peel and flesh of apple that differentiate the wild germplasm, advanced selections and F1 segregating population.Table S2. Putative metabolites in peel having significantly higher contents in some of the progeny than both the parents of the segregating population.
ArticleID:PBR12134
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content type line 23
ISSN:0179-9541
1439-0523
DOI:10.1111/pbr.12134