Characterization of amino acid transport in the dairy strain Leuconostoc mesenteroides subsp. mesenteroides CNRZ 1273

• Published in: Letters in applied microbiology Vol. 35; no. 4; pp. 291 - 295
• Main Authors: Gendrot, F., Foucaud‐Scheunemann, C., Ferchichi, M., Hemme, D.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.2002; Blackwell Science
• Subjects: Amino Acids - metabolism; Animals; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Culture Media; Food industries; Fundamental and applied biological sciences. Psychology; Kinetics; Leuconostoc - metabolism; Milk - microbiology; Milk and cheese industries. Ice creams; Mission oriented research; Physiology and metabolism; Protein Transport; Valine - metabolism
• ISSN: 0266-8254; 1472-765X; 1365-2673
• DOI: 10.1046/j.1472-765X.2002.01191.x

Aims: To identify and characterize amino acid transport in Leuconostoc mesenteroides. 
Methods and Results: The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transport. Some of the systems were shared by several amino acids. Kinetic analysis indicated the presence of three transport systems with very high (VH), high (H) and low affinity (H) for the 11 amino acids studied. The Kt values (µmol l−1) ranged from 0·088 to 0·815 (VH), 6–390 (H) and 320–4500 (L) and the Vmax values [nmol s−1 (g dry weight)−1] from 0·015 to 0·8 (VH), 15–95 (H) and 90–470 (L). 
Conclusions: The study showed the presence of three transport systems in Leuc. mesenteroides for all amino acids tested, some of them being shared by several amino acids. 
Significance and Impact of the Study. The findings are discussed with reference to the growth of Leuc. mesenteroides in milk as pure or in mixed‐strain culture with Lactococcus lactis.