Studies on Pharmacological Activation of Human Immunoglobulin G by Chemical Modification and Active Subfragments. XII. Effect of Carboxamidemethylated Fc Fragment (CM-Fc) from Human Immunoglobulin G on Mixed Lymphocyte Reaction

• Published in: Biological & pharmaceutical bulletin Vol. 17; no. 3; pp. 411 - 414
• Main Authors: MIMURA, Tsutomu, ITOH, Masaaki, SUGIYAMA, Tetsuya, SUZUKI, Nobutaka, NAKANISHI, Tsuyoshi, TSUJIKAWA, Kazutake, KOHAMA, Yasuhiro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1994; Maruzen; Japan Science and Technology Agency
• Subjects: Animals; Biological and medical sciences; carboxamidemethylated Fc (CM-Fc); Cell Division - drug effects; Cytokines - pharmacology; Dinoprostone - biosynthesis; Humans; IL-1; Immunoglobulin Fc Fragments - chemistry; Immunoglobulin Fc Fragments - pharmacology; Immunoglobulin G - biosynthesis; Immunoglobulin G - chemistry; Immunoglobulin G - pharmacology; Immunomodulators; Indomethacin - pharmacology; Interleukin-1 - metabolism; Lymphocyte Culture Test, Mixed; macrophage; Male; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; mixed lymphocyte reaction (MLR); Pharmacology. Drug treatments; prostaglandin E2; Spleen - cytology; TNF; Tumor Necrosis Factor-alpha - metabolism; Human; Immunoglobulins; Mixed lymphocyte reaction; Fc-Fragment; Structure activity relation; Immunomodulation; IgG; Carboxamidomethylation; In vitro; Biological activity; Macrophage
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.17.411

The effect of carboxamidemethylated Fc fragment (CM-Fc) from human serum immunoglobulin G on mixed lymphocyte reaction (MLR) was studied. CM-Fc enhanced the MLR response when added at the start of cultivation, but suppressed the response when added 48 h after onset of cultivation. The Fc fragment had no effect. CM-Fc elevated the levels of interleukin-1 and tumor necrosis factor when added at the start of splenocyte cultivation, and somewhat did so when added 48 h after onset of cultivation. These elevations caused the induction of prostaglandin E2 in MLR culture supernatant. The suppression of MLR by CM-Fc was abrogated by the addition of indomethacin. We considered that CM-Fc regulated the immune response being affected to the function of macrophages.