Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport

• Published in: Angewandte Chemie International Edition Vol. 60; no. 9; pp. 4907 - 4914
• Main Authors: Ma, Cheng, Wu, Shaojun, Zhou, Yang, Wei, Hui‐Fang, Zhang, Jianrong, Chen, Zixuan, Zhu, Jun‐Jie, Lin, Yuehe, Zhu, Wenlei
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 23.02.2021
• Edition: International ed. in English
• Subjects: Actin; Animals; Autophagy; bioelectrochemistry; Biomolecules; Cell membranes; Cytoskeleton; Damage detection; DNA damage; DNA Damage - drug effects; Edge effect; Electrochemical Techniques; Electrochemiluminescence; Electrochemistry; Electrodes; Electroporation; HeLa Cells; Humans; Image enhancement; Image processing; Intracellular; Liver - microbiology; Liver - pathology; Luminescent Measurements; Mice; Microscopy; Microscopy, Atomic Force; Microscopy, Fluorescence - methods; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Phagocytosis; Reactive Oxygen Species - metabolism; Shewanella - isolation & purification; Single-Cell Analysis; single-cell studies; electrochemiluminescence; bioelectrochemistry; microscopy; electrochemistry; single-cell studies
• ISSN: 1433-7851; 1521-3773; 1521-3773
• DOI: 10.1002/anie.202012171

A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR‐1. Amine‐rich biomolecules as consumed coreactants drive electrochemiluminescence with Ru(bpy)32+, enabling bio‐coreactant‐enhanced single‐cell electrochemiluminescence microscopy. This allows the imaging of intracellular hierarchical structures without the use of multiple labels. Dynamic signals disclose the universal edge effect of cellular electroporation and enable the visualization of heterogeneous molecular transport.