Molybdenum and phosphorus limitation of moss-associated nitrogen fixation in boreal ecosystems

• Published in: The New phytologist Vol. 214; no. 1; pp. 97 - 107
• Main Authors: Rousk, Kathrin, Degboe, Jefferson, Michelsen, Anders, Bradley, Robert, Bellenger, Jean‐Philippe
• Format: Journal Article
• Language: English
• Published: England New Phytologist Trust 01.04.2017; Wiley Subscription Services, Inc
• Subjects: Acetylene - metabolism; Acetylene reduction; biological nitrogen fixation; Biomass; Boreal forests; Bryophyta - physiology; Cyanobacteria; Cyanobacteria - drug effects; Cyanobacteria - metabolism; Ecological function; Ecosystem; Ecosystems; field experimentation; Field tests; Growing season; Hylocomium splendens; latitude; Molybdenum; molybdenum (Mo); Molybdenum - pharmacology; Mosses; mosses and liverworts; nitrogen; Nitrogen fixation; Nitrogen Fixation - drug effects; Nitrogen Isotopes; nitrogenase; nutrients; phosphorus; phosphorus (P); Phosphorus - pharmacology; Picea; Pleurozium schreberi; stable isotopes; subarctic tundra; Tracer techniques; Tundra; boreal forests; cyanobacteria; subarctic tundra; biological nitrogen fixation; molybdenum (Mo); Hylocomium splendens; Pleurozium schreberi; phosphorus (P)
• ISSN: 0028-646X; 1469-8137; 1469-8137
• DOI: 10.1111/nph.14331

Biological nitrogen fixation (BNF) performed by moss-associated cyanobacteria is one of the main sources of new nitrogen (N) input in pristine, high-latitude ecosystems. Yet, the nutrients that limit BNF remain elusive. Here, we tested whether this important ecosystem function is limited by the availability of molybdenum (Mo), phosphorus (P), or both. BNF in dominant mosses was measured with the acetylene reduction assay (ARA) at different time intervals following Mo and P additions, in both laboratory microcosms with mosses from a boreal spruce forest and field plots in subarctic tundra. We further used a 15N2 tracer technique to assess the ARA to N2 fixation conversion ratios at our subarctic site. BNF was up to four-fold higher shortly after the addition of Mo, in both the laboratory and field experiments. A similar positive response to Mo was found in moss colonizing cyanobacterial biomass. As the growing season progressed, nitrogenase activity became progressively more P limited. The ARA: 15N2 ratios increased with increasing Mo additions. These findings show that N2 fixation activity as well as cyanobacterial biomass in dominant feather mosses from boreal forests and subarctic tundra are limited by Mo availability.