T cell–derived exosomes induced macrophage inflammatory protein‐1α/β drive the trafficking of CD8+ T cells in oral lichen planus

• Published in: Journal of cellular and molecular medicine Vol. 24; no. 23; pp. 14086 - 14098
• Main Authors: Yang, Jing‐Ya, Zhang, Jing, Lu, Rui, Tan, Ya‐Qin, Du, Ge‐Fei, Zhou, Gang
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.12.2020; John Wiley and Sons Inc
• Subjects: Adult; Antibodies; Antigens; Biomarkers; Case-Control Studies; CC chemokine receptors; CCR1 protein; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; Cell Movement - immunology; Chemokine CCL3 - genetics; Chemokine CCL3 - metabolism; Chemokine CCL4 - genetics; Chemokine CCL4 - metabolism; Chemokines; Cytokines; Cytokines - genetics; Cytokines - metabolism; Cytotoxicity; Disease Susceptibility; Enzyme-linked immunosorbent assay; exosome; Exosomes; Exosomes - metabolism; Female; Flow cytometry; Gene Expression; Humans; Immunohistochemistry; Inflammation; Inflammatory diseases; Leukocyte migration; Leukocytes (mononuclear); Lichen planus; Lichen Planus, Oral - etiology; Lichen Planus, Oral - metabolism; Lichen Planus, Oral - pathology; Lymphocytes; Lymphocytes T; Macrophage inflammatory protein; macrophage inflammatory protein‐1α/β; Male; Middle Aged; migration; oral lichen planus; Original; Polymerase chain reaction; Proteins; Severity of Illness Index; T cell; migration; macrophage inflammatory protein-1α/β; oral lichen planus; exosome; T cell
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.16020

Oral lichen planus (OLP) is a T cell–mediated chronic inflammatory disease with uncertain aetiology. Exosomes are nanosized particles with biological capacities. Here, we aimed to study the effects of T cell–derived exosomes (T‐exos) on the pathogenesis of OLP and its mechanism. T‐exos were incubated with Jurkat cells for 48 hours, and 26 cytokines in the supernatant were measured by luminex assay. The expression of macrophage inflammatory protein (MIP)‐1α/β was detected using immunohistochemistry and ELISA; that of CCR1/3/5 on peripheral T cells was determined by flow cytometry. Transwell assay was performed to investigate the chemotactic effect of MIP‐1α/β, and cells in the lower chambers were examinated by flow cytometry. As a result, OLP T‐exos elevated the production of MIP‐1α/β, which were highly expressed in OLP tissues and plasma. CCR1/5 were markedly expressed on OLP peripheral T cells, and the majority of CCR1/5+ T cells were CD8+ T cells. Besides, MIP‐1α/β promoted the migration of OLP mononuclear cells, while inhibiting CCR1/5 significantly decreased the trafficking of mononuclear cells, especially that of CD8+ T cells. Conclusively, OLP T‐exos‐induced MIP‐1α/β may drive the trafficking of CD8+ T cells after binding with CCR1/5 in OLP, contributing to the development of OLP.